GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00001000
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait
Trait State in Taxon A
Arabidopsis thaliana sensitive to Pseudomonas syringae pathovar maculicola
Trait State in Taxon B
Arabidopsis thaliana Col-0 resistant to Pseudomonas syringae pathovar maculicola
Ancestral State
Unknown
Taxonomic Status
Taxon A
Latin Name
Common Name
thale cress
Synonyms
thale cress; mouse-ear cress; thale-cress; Arabidopsis thaliana (L.) Heynh.; Arabidopsis thaliana (thale cress); Arabidopsis_thaliana; Arbisopsis thaliana; thale kress
Rank
species
Lineage
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; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
thale cress
Synonyms
thale cress; mouse-ear cress; thale-cress; Arabidopsis thaliana (L.) Heynh.; Arabidopsis thaliana (thale cress); Arabidopsis_thaliana; Arbisopsis thaliana; thale kress
Rank
species
Lineage
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; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Arabidopsis thaliana Col-0 (resistant)
GENOTYPIC CHANGE
Generic Gene Name
RPM1
Synonyms
DISEASE RESISTANCE PROTEIN RPM1; RESISTANCE TO P. SYRINGAE PV MACULICOLA 1; RESISTANCE TO PSEUDOMONAS SYRINGAE 3; RPS3; At3g07040; F17A9.20
String
Sequence Similarities
Belongs to the disease resistance NB-LRR family.
GO - Molecular Function
GO:0005524 : ATP binding
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GO - Biological Process
GO - Cellular Component
GO:0005886 : plasma membrane
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Presumptive Null
Molecular Type
Aberration Type
Indel Size
1-10 kb
Molecular Details of the Mutation
3.7kb indel with a null-state in the sensitive strains
Experimental Evidence
Main Reference
Authors
Grant MR; McDowell JM; Sharpe AG; de Torres Zabala M; Lydiate DJ; Dangl JL
Abstract
Plant disease resistance (R) genes confer race-specific resistance to pathogens and are genetically defined on the basis of intra-specific functional polymorphism. Little is known about the evolutionary mechanisms that generate this polymorphism. Most R loci examined to date contain alternate alleles and/or linked homologs even in disease-susceptible plant genotypes. In contrast, the resistance to Pseudomonas syringae pathovar maculicola (RPM1) bacterial resistance gene is completely absent (rpm1-null) in 5/5 Arabidopsis thaliana accessions that lack RPM1 function. The rpm1-null locus contains a 98-bp segment of unknown origin in place of the RPM1 gene. We undertook comparative mapping of RPM1 and flanking genes in Brassica napus to determine the ancestral state of the RPM1 locus. We cloned two B. napus RPM1 homologs encoding hypothetical proteins with approximately 81% amino acid identity to Arabidopsis RPM1. Collinearity of genes flanking RPM1 is conserved between B. napus and Arabidopsis. Surprisingly, we found four additional B. napus loci in which the flanking marker synteny is maintained but RPM1 is absent. These B. napus rpm1-null loci have no detectable nucleotide similarity to the Arabidopsis rpm1-null allele. We conclude that RPM1 evolved before the divergence of the Brassicaceae and has been deleted independently in the Brassica and Arabidopsis lineages. These results suggest that functional polymorphism at R gene loci can arise from gene deletions.
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