GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001102
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
food shortage accelerates development
Trait State in Taxon B
food shortage has no effect on developmental time. Synchronization of individuals reared in mass population.
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
domestic silkworm
Synonyms
domestic silkworm; silk moth; silkworm; Bombyx mori Linnaeus, 1758
Rank
species
Lineage
Show more ... xapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Bombycoidea; Bombycidae; Bombycinae; Bombyx
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
domestic silkworm
Synonyms
domestic silkworm; silk moth; silkworm; Bombyx mori Linnaeus, 1758
Rank
species
Lineage
Show more ... xapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Bombycoidea; Bombycidae; Bombycinae; Bombyx
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
Eo
Synonyms
CG9504; Dmel\CG9504; DmEO; EO; EObeta1; Eo-RA; Dmel_CG9504
Sequence Similarities
-
UniProtKB
Drosophila melanogaster
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Insertion Size
100-999 bp
Molecular Details of the Mutation
insertion of a 512bp fragment of a Taguchi transposable element 462 bp upstream of the transcription start site of the EO gene. The TE insertion enhances the transcription of flanking genes after 20-hydroxyecdysone treatment.
Experimental Evidence
Authors
Sun W; Shen YH; Han MJ; Cao YF; Zhang Z
Abstract
Although there are many studies to show a key role of transposable elements (TEs) in adaptive evolution of higher organisms, little is known about the molecular mechanisms. In this study, we found that a partial TE (Taguchi) inserted in the cis-regulatory region of the silkworm ecdysone oxidase (EO) gene, which encodes a crucial enzyme to reduce the titer of molting hormone (20-hydroxyecdysone, 20E). The TE insertion occurred during domestication of silkworm and the frequency of the TE insertion in the domesticated silkworm (Bombyx mori) is high, 54.24%. The linkage disequilibrium in the TE inserted strains of the domesticated silkworm was elevated. Molecular population genetics analyses suggest that this TE insertion is adaptive for the domesticated silkworm. Luminescent reporter assay shows that the TE inserted in the cis-regulatory region of the EO gene functions as a 20E-induced enhancer of the gene expression. Further, phenotypic bioassay indicates that the silkworm with the TE insertion exhibited more stable developmental phenotype than the silkworm without the TE insertion when suffering from food shortage. Thus, the inserted TE in the cis-regulatory region of the EO gene increased developmental uniformity of silkworm individuals through regulating 20E metabolism, partially explaining transformation of a domestication developmental trait in the domesticated silkworm. Our results emphasize the exceptional role of gene expression regulation in developmental transition of domesticated animals.

© The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
COMMENTS
Food shortage leads to higher levels of ecdysone in both types of individuals. This leads to higher levels of ecdysone oxidase in individuas carrying the TE (Taxon B) compared to the ones without TE insertion (Taxon A); and thus back to normal ecdysone levels and no accelerated development.
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