GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00001195
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Vitis vinifera - Syrah
Trait State in Taxon B
Vitis vinifera - Grenache with white berries
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
wine grape
Synonyms
Vitis vinifera subsp. vinifera; wine grape; Vitis vinifera L.
Rank
species
Lineage
Show more ... tina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; rosids incertae sedis; Vitales; Vitaceae; Viteae; Vitis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Vitis vinifera - Syrah
Taxon B
Latin Name
Common Name
wine grape
Synonyms
Vitis vinifera subsp. vinifera; wine grape; Vitis vinifera L.
Rank
species
Lineage
Show more ... tina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; rosids incertae sedis; Vitales; Vitaceae; Viteae; Vitis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Vitis vinifera - Grenache
GENOTYPIC CHANGE
UniProtKB
Vitis vinifera
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Indel Size
unknown
Molecular Details of the Mutation
Frameshift leading to a truncated 265-amino-acid protein instead of 344
Experimental Evidence
Authors
Fournier-Level A; Le Cunff L; Gomez C; Doligez A; Ageorges A; Roux C; Bertrand Y; Souquet JM; et al. ... show more
Abstract
The combination of QTL mapping studies of synthetic lines and association mapping studies of natural diversity represents an opportunity to throw light on the genetically based variation of quantitative traits. With the positional information provided through quantitative trait locus (QTL) mapping, which often leads to wide intervals encompassing numerous genes, it is now feasible to directly target candidate genes that are likely to be responsible for the observed variation in completely sequenced genomes and to test their effects through association genetics. This approach was performed in grape, a newly sequenced genome, to decipher the genetic architecture of anthocyanin content. Grapes may be either white or colored, ranging from the lightest pink to the darkest purple tones according to the amount of anthocyanin accumulated in the berry skin, which is a crucial trait for both wine quality and human nutrition. Although the determinism of the white phenotype has been fully identified, the genetic bases of the quantitative variation of anthocyanin content in berry skin remain unclear. A single QTL responsible for up to 62% of the variation in the anthocyanin content was mapped on a Syrah x Grenache F(1) pseudo-testcross. Among the 68 unigenes identified in the grape genome within the QTL interval, a cluster of four Myb-type genes was selected on the basis of physiological evidence (VvMybA1, VvMybA2, VvMybA3, and VvMybA4). From a core collection of natural resources (141 individuals), 32 polymorphisms revealed significant association, and extended linkage disequilibrium was observed. Using a multivariate regression method, we demonstrated that five polymorphisms in VvMybA genes except VvMybA4 (one retrotransposon, three single nucleotide polymorphisms and one 2-bp insertion/deletion) accounted for 84% of the observed variation. All these polymorphisms led to either structural changes in the MYB proteins or differences in the VvMybAs promoters. We concluded that the continuous variation in anthocyanin content in grape was explained mainly by a single gene cluster of three VvMybA genes. The use of natural diversity helped to reduce one QTL to a set of five quantitative trait nucleotides and gave a clear picture of how isogenes combined their effects to shape grape color. Such analysis also illustrates how isogenes combine their effect to shape a complex quantitative trait and enables the definition of markers directly targeted for upcoming breeding programs.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
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