GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00001439
Main curator
Prigent
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Spodoptera exigua (FRA) from France susceptible
Trait State in Taxon B
Spodoptera exigua (Xen-R) highly resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
beet armyworm
Synonyms
beet armyworm; pigweed caterpillar; small mottled willow caterpillar; Spodoptera exigua (Hubner, 1808)
Rank
species
Lineage
Show more ... da; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Amphipyrinae; Spodoptera
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
beet armyworm
Synonyms
beet armyworm; pigweed caterpillar; small mottled willow caterpillar; Spodoptera exigua (Hubner, 1808)
Rank
species
Lineage
Show more ... da; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Amphipyrinae; Spodoptera
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
-
Synonyms
-
String
-
Sequence Similarities
-
GO - Molecular Function
-
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Deletion Size
100-999 bp
Molecular Details of the Mutation
About 500 nt genomic deletion over 2 exons involving 246 nt coding 82aa
Experimental Evidence
Authors
Park Y; González-Martínez RM; Navarro-Cerrillo G; Chakroun M; Kim Y; Ziarsolo P; Blanca J; Cañizares J; et al. ... show more
Abstract
Relatively recent evidence indicates that ABCC2 transporters play a main role in the mode of action of Bacillus thuringiensis (Bt) Cry1A-type proteins. Mapping of major Cry1A resistance genes has linked resistance to the ABCC2 locus in Heliothis virescens, Plutella xylostella, Trichoplusia ni and Bombyx mori, and mutations in this gene have been found in three of these Bt-resistant strains.

We have used a colony of Spodoptera exigua (Xen-R) highly resistant to a Bt commercial bioinsecticide to identify regions in the S. exigua genome containing loci for major resistance genes by using bulk segregant analysis (BSA). Results reveal a region containing three genes from the ABCC family (ABBC1, ABBC2 and ABBC3) and a mutation in one of them (ABBC2) as responsible for the resistance of S. exigua to the Bt commercial product and to its key Spodoptera-active ingredients, Cry1Ca. In contrast to all previously described mutations in ABCC2 genes that directly or indirectly affect the extracellular domains of the membrane protein, the ABCC2 mutation found in S. exigua affects an intracellular domain involved in ATP binding. Functional analyses of ABBC2 and ABBC3 support the role of both proteins in the mode of action of Bt toxins in S. exigua. Partial silencing of these genes with dsRNA decreased the susceptibility of wild type larvae to both Cry1Ac and Cry1Ca. In addition, reduction of ABBC2 and ABBC3 expression negatively affected some fitness components and induced up-regulation of arylphorin and repat5, genes that respond to Bt intoxication and that are found constitutively up-regulated in the Xen-R strain.

The current results show the involvement of different members of the ABCC family in the mode of action of B. thuringiensis proteins and expand the role of the ABCC2 transporter in B. thuringiensis resistance beyond the Cry1A family of proteins to include Cry1Ca.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
COMMENTS
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