GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001474
Main curator
Prigent
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Peach-potato aphid 4106A does not survive for 144h on a diet containing 30ppm nicotine ; tobacco aphid JR with 100% mortality at 144h on a diet containing 320ppm nicotine
Trait State in Taxon B
tobacco aphid 5410R with 7% mortality at 144h on a diet containing 320ppm nicotine ; tobacco aphid 5191A survive for 144h on a diet containing 30ppm nicotine
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
green peach aphid
Synonyms
Myzus (Nectarosiphon) persicae; green peach aphid; peach-potato aphid; Myzus persicae (Sulzer, 1776); Myzus persiceae
Rank
species
Lineage
Show more ... Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae; Aphidinae; Macrosiphini; Myzus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Peach-potato aphid 4106A does not survive for 144h on a diet containing 30ppm nicotine ; tobacco aphid JR with 100% mortality at 144h on a diet containing 320ppm nicotine
Taxon B
Latin Name
Common Name
green peach aphid
Synonyms
Myzus (Nectarosiphon) persicae; green peach aphid; peach-potato aphid; Myzus persicae (Sulzer, 1776); Myzus persiceae
Rank
species
Lineage
Show more ... Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae; Aphidinae; Macrosiphini; Myzus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
tobacco aphid 5410R with 7% mortality at 144h on a diet containing 320ppm nicotine ; tobacco aphid 5191A survive for 144h on a diet containing 30ppm nicotine
GENOTYPIC CHANGE
UniProtKB
Myzus persicae
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Insertion Size
10-99 bp
Molecular Details of the Mutation
Expansion of a AC dinucleotide microsatellite (from 15 to 48 repeat units) in the promoter 198 bp upstream of the start codon that enhances gene expression
Experimental Evidence
Authors
Bass C; Zimmer CT; Riveron JM; Wilding CS; Wondji CS; Kaussmann M; Field LM; Williamson MS; et al. ... show more
Abstract
Host plant shifts of herbivorous insects may be a first step toward sympatric speciation and can create new pests of agriculturally important crops; however, the molecular mechanisms that mediate this process are poorly understood. Certain races of the polyphagous aphid Myzus persicae have recently adapted to feed on tobacco (Myzus persicae nicotianae) and show a reduced sensitivity to the plant alkaloid nicotine and cross-resistance to neonicotinoids a class of synthetic insecticides widely used for control. Here we show constitutive overexpression of a cytochrome P450 (CYP6CY3) allows tobacco-adapted races of M. persicae to efficiently detoxify nicotine and has preadapted them to resist neonicotinoid insecticides. CYP6CY3, is highly overexpressed in M. persicae nicotianae clones from three continents compared with M. persicae s.s. and expression level is significantly correlated with tolerance to nicotine. CYP6CY3 is highly efficient (compared with the primary human nicotine-metabolizing P450) at metabolizing nicotine and neonicotinoids to less toxic metabolites in vitro and generation of transgenic Drosophila expressing CYP6CY3 demonstrate that it confers resistance to both compounds in vivo. Overexpression of CYP6CY3 results from the expansion of a dinucleotide microsatellite in the promoter region and a recent gene amplification, with some aphid clones carrying up to 100 copies. We conclude that the mutations leading to overexpression of CYP6CY3 were a prerequisite for the host shift of M. persicae to tobacco and that gene amplification and microsatellite polymorphism are evolutionary drivers in insect host adaptation.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
1
COMMENTS
For four of the M. persicae nicotianae clones all amplified copies of CYP6CY3 appear to share the microsatellite expansion suggests that this mutation predates the gene amplification event ; an other A>G SNP 138 bp upstream of the start codon is also observed but it is experimentally shown to not be involved
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