GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001601
Main curator
Prigent
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Plutella xylostella BCS-S and Japan reference strains susceptible
Trait State in Taxon B
Plutella xylostella Chinese and Indian field strains
Ancestral State
Unknown
Taxonomic Status
Taxon A
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Plutella xylostella BCS-S and Japan reference strains susceptible
Taxon B
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Plutella xylostella Chinese and Indian field strains
GENOTYPIC CHANGE
Generic Gene Name
CHS1
Synonyms
CHS1; PxCHS1B
String
-
Sequence Similarities
-
GO - Biological Process
-
UniProtKB
Plutella xylostella
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
A>T p.I1042F (I1056 in D. melanogaster) located in the C-terminal transmembrane domain
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Douris V; Steinbach D; Panteleri R; Livadaras I; Pickett JA; Van Leeuwen T; Nauen R; Vontas J
Abstract
Despite the major role of chitin biosynthesis inhibitors such as benzoylureas (BPUs) in the control of pests in agricultural and public health for almost four decades, their molecular mode of action (MoA) has in most cases remained elusive. BPUs interfere with chitin biosynthesis and were thought to interact with sulfonylurea receptors that mediate chitin vesicle transport. Here, we uncover a mutation (I1042M) in the chitin synthase 1 (CHS1) gene of BPU-resistant Plutella xylostella at the same position as the I1017F mutation reported in spider mites that confers etoxazole resistance. Using a genome-editing CRISPR/Cas9 approach coupled with homology-directed repair (HDR) in Drosophila melanogaster, we introduced both substitutions (I1056M/F) in the corresponding fly CHS1 gene (kkv). Homozygous lines bearing either of these mutations were highly resistant to etoxazole and all tested BPUs, as well as buprofezin-an important hemipteran chitin biosynthesis inhibitor. This provides compelling evidence that BPUs, etoxazole, and buprofezin share in fact the same molecular MoA and directly interact with CHS. This finding has immediate effects on resistance management strategies of major agricultural pests but also on mosquito vectors of serious human diseases such as Dengue and Zika, as diflubenzuron, the standard BPU, is one of the few effective larvicides in use. The study elaborates on how genome editing can directly, rapidly, and convincingly elucidate the MoA of bioactive molecules, especially when target sites are complex and hard to reconstitute in vitro.
Additional References
RELATED GEPHE
Related Haplotypes
1
EXTERNAL LINKS
COMMENTS
transgenic flies with the same mutation is highly resistant to etoxazole and all tested benzoylureas as well as buprofezin
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