GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001602
Main curator
Prigent
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
spider mite Tetranychus urticae susceptible
Trait State in Taxon B
spider mite Tetranychus urticae strain HexR and strain005 hexythiazox clofentezine and etoxazole-resistant
Ancestral State
Unknown
Taxonomic Status
Taxon A
Common Name
two-spotted spider mite
Synonyms
two-spotted spider mite; red spider mite; twospotted mite; Tetranychus urticae Koch, 1836
Rank
species
Lineage
Show more ... ia; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
spider mite Tetranychus urticae susceptible
Taxon B
Common Name
two-spotted spider mite
Synonyms
two-spotted spider mite; red spider mite; twospotted mite; Tetranychus urticae Koch, 1836
Rank
species
Lineage
Show more ... ia; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
spider mite Tetranychus urticae strain HexR and strain005 hexythiazox clofentezine and etoxazole-resistant
GENOTYPIC CHANGE
Generic Gene Name
chs1
Synonyms
chs1; 107359084
String
-
Sequence Similarities
-
GO - Biological Process
-
UniProtKB
Tetranychus urticae
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
A>T p.I1017F (I1056 in D. melanogaster) located in the C-terminal transmembrane domain
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Demaeght P; Osborne EJ; Odman-Naresh J; Grbić M; Nauen R; Merzendorfer H; Clark RM; Van Leeuwen T
Abstract
The acaricides clofentezine, hexythiazox and etoxazole are commonly referred to as 'mite growth inhibitors', and clofentezine and hexythiazox have been used successfully for the integrated control of plant mite pests for decades. Although they are still important today, their mode of action has remained elusive. Recently, a mutation in chitin synthase 1 (CHS1) was linked to etoxazole resistance. In this study, we identified and investigated a Tetranychus urticae strain (HexR) harboring recessive, monogenic resistance to each of hexythiazox, clofentezine, and etoxazole. To elucidate if there is a common genetic basis for the observed cross-resistance, we adapted a previously developed bulk segregant analysis method to map with high resolution a single, shared resistance locus for all three compounds. This finding indicates that the underlying molecular basis for resistance to all three compounds is identical. This locus is centered on the CHS1 gene, and as supported by additional genetic and biochemical studies, a non-synonymous variant (I1017F) in CHS1 associates with resistance to each of the tested acaricides in HexR. Our findings thus demonstrate a shared molecular mode of action for the chemically diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole as inhibitors of an essential, non-catalytic activity of CHS1. Given the previously documented cross-resistance between clofentezine, hexythiazox and the benzyolphenylurea (BPU) compounds flufenoxuron and cycloxuron, CHS1 should be also considered as a potential target-site of insecticidal BPUs.

Copyright © 2014 Elsevier Ltd. All rights reserved.
RELATED GEPHE
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
Observed pattern is indicative of recurrent mutation and selection in EtoxR and Strain005. Transgenic flies with the same mutation is highly resistant to etoxazole and all tested benzoylureas as well as buprofezin
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