GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001673
Main curator
Prigent
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Bt-susceptible
Trait State in Taxon B
Bt-resistant strains collected from Florida (DBM1Ac-R >3500-fold resistance) Shenzhen (SZ-R 458-fold CryAc resistance) Shanghai (SH-R 1890-fold Bt var. kurstaki resistance) and NIL-R (>3900-fold Cry1Ac resistance and >2800-fold Btk resistance)
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Diamondback moth Bt susceptible strain DBM1Ac-S
Taxon B
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Diamondback moth strains collected from Florida (DBM1Ac-R >3500-fold resistance) Shenzhen (SZ-R 458-fold CryAc resistance) Shanghai (SH-R 1890-fold Bt var. kurstaki resistance) and NIL-R (>3900-fold Cry1Ac resistance and >2800-fold Btk resistance)
GENOTYPIC CHANGE
Generic Gene Name
MAP4K4
Synonyms
HGK; NIK; MEKKK4; FLH21957; HEL-S-31; KIAA0687
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
UniProtKB
Homo sapiens
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
MAP4K4 constitutively up-regulated leading to up-regulated PxABCC1 and down-regulated PxABCC2 and PxABCC3 and reduction of membrane-bound ALP (mALP) expression responsible for reduced Cry1Ac toxin binding to midgut proteins
Experimental Evidence
Authors
Guo Z; Kang S; Chen D; Wu Q; Wang S; Xie W; Zhu X; Baxter SW; et al. ... show more
Abstract
Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously mapped to a multigenic resistance locus (BtR-1). Here, we assembled the 3.15 Mb BtR-1 locus and found high-level resistance to Cry1Ac and Bt biopesticide in four independent P. xylostella strains were all associated with differential expression of a midgut membrane-bound alkaline phosphatase (ALP) outside this locus and a suite of ATP-binding cassette transporter subfamily C (ABCC) genes inside this locus. The interplay between these resistance genes is controlled by a previously uncharacterized trans-regulatory mechanism via the mitogen-activated protein kinase (MAPK) signaling pathway. Molecular, biochemical, and functional analyses have established ALP as a functional Cry1Ac receptor. Phenotypic association experiments revealed that the recessive Cry1Ac resistance was tightly linked to down-regulation of ALP, ABCC2 and ABCC3, whereas it was not linked to up-regulation of ABCC1. Silencing of ABCC2 and ABCC3 in susceptible larvae reduced their susceptibility to Cry1Ac but did not affect the expression of ALP, whereas suppression of MAP4K4, a constitutively transcriptionally-activated MAPK upstream gene within the BtR-1 locus, led to a transient recovery of gene expression thereby restoring the susceptibility in resistant larvae. These results highlight a crucial role for ALP and ABCC genes in field-evolved resistance to Cry1Ac and reveal a novel trans-regulatory signaling mechanism responsible for modulating the expression of these pivotal genes in P. xylostella.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
Possible Supergene since the mapped interval contains toxin receptors in addition to the upstream regulator. RNAi evidence seems to validate the MAPK gene
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