GEPHE SUMMARY Print
cellular organisms; Eukaryota; Opisthokonta; Metazoa
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Hydra attenuata; Hydra magnipapillata; Hydra magnipapillata Ito, 1947; Hydra vulgaris Pallas, 1766
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata; Aplanulata; Hydridae; Hydra
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Generic Gene Name
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
GO - Molecular Function
GO:0005524 : ATP binding ... show more
GO - Biological Process
GO - Cellular Component
GO:0016021 : integral component of membrane ... show more
Molecular Details of the Mutation
Canfield VA; Xu KY; D'Aquila T; Shyjan AW; Levenson R
We have used molecular and biochemical techniques to analyze Na,K-ATPase from a simple metazoan, Hydra vulgaris. First we isolated and characterized cDNA clones encoding the Na,K-ATPase alpha subunit from a Hydra lambda gt11 cDNA library. The open reading frame predicts a protein of 1031 amino acids that bears a high degree of primary sequence and secondary structure similarity to mammalian, avian, and arthropod alpha subunits. The predicted Hydra alpha subunit contains charged residues at the termini of the H1-H2 extracellular domain, suggesting that the Hydra alpha subunit may be resistant to cardiac glycoside inhibition. Biochemical analysis of partially purified Hydra Na,K-ATPase reveals both high- and low-affinity components of ouabain-inhibitable ATPase activity. Our results suggest that the evolutionary ancestor of all metazoans possessed a Na,K-ATPase alpha subunit that was highly conserved with respect to its vertebrate counterparts. Further, expression of a ouabain-resistant Na,K-ATPase activity in Hydra suggests that cardiac glycoside resistance arose randomly during evolution of the Na,K-ATPase.
59 (ABCA2, BTR1- Cadherin-like protein, Ha_BtR, ABCC2, para (kdr), Chitin synthase 1 (CHS1), MAP4K4, Acetylcholinesterase (Ace-1), resistance to dieldrin, esterase B1, Acetylcholinesterase (Ace-2), alcohol dehydrogenase (Adh), Aldehyde dehydrogenase (Aldh), CG11699, Cyp12d1, Cyp28d1, cyp6d2, cyp6g1, GSTE1-E10 cluster, kin of irre (kire), PHGPx, RnrS, SOD1, Ugt86Dd, Acetylcholinesterase (Ace), esterase isozyme E7 = E3, esterase E4, AHR, Vkorc1, SCN9A (Nav1.7), AHR2, AIP, alcohol dehydrogenase (ADH1B), CYP2C9, CYP4F2, MDR1, beta-tubulin, beta-tubulin (ben-1), GLC-1, str-217, CHRNA1, CYP6AB3, CYP6P9 cluster (CYP6P9a and CYP6P9b), CYP6P9; CYP6P4 cluster, esterase B1 + esterase A, esterase B1 = esterase beta1, esterase isozyme E3, Nav1.6 sodium channel, Nav1.7 sodium channel, SCN4A (Nav1.4), SCN8A (Nav1.6), SCN4A (Nav1.4a gene copy), SCN4A (Nav1.4b gene copy), Sulfotransferase-OXA-Resistance (SULT-OR), CHKov1, CYP6B1, CYP6B4, FMO1, CYP6CY3)
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