GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000176
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Hordeum vulgare; Spring "Tremois"
Trait State in Taxon B
Hordeum vulgare; winter-hardy "Dicktoo" and "Nure"
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Common Name
-
Synonyms
barley; Hordeum vulgare L.; Horedum vulgare
Rank
species
Lineage
Show more ... yta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Hordeum vulgare; Tremois
Taxon B
Common Name
-
Synonyms
barley; Hordeum vulgare L.; Horedum vulgare
Rank
species
Lineage
Show more ... yta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Hordeum vulgare; Barley genotypes "Dicktoo" and "Nure"
GENOTYPIC CHANGE
UniProtKB
Triticum monococcum
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
tandem duplication of CBF2-CBF4 region and/or pseudogenisation of CBF13
Experimental Evidence
Authors
Knox AK; Dhillon T; Cheng H; Tondelli A; Pecchioni N; Stockinger EJ
Abstract
Frost Resistance-1 (FR-1) and FR-2 are two loci affecting freezing tolerance and winter hardiness of the temperate-climate cereals. FR-1 is hypothesized to be due to the pleiotropic effects of VRN-1. FR-2 spans a cluster of C-Repeat Binding Factor (CBF) genes. These loci are genetically and functionally linked. Recent studies indicate CBF transcripts are downregulated by the VRN-1 encoded MADS-box protein or a factor in the VRN-1 pathway. Here, we report that barley genotypes 'Dicktoo' and 'Nure' carrying a vrn-H1 winter allele at VRN-H1 harbor increased copy numbers of CBF coding sequences relative to Vrn-H1 spring allele genotypes 'Morex' and 'Tremois'. Sequencing bacteriophage lambda genomic clones from these four genotypes alongside DNA blot hybridizations indicate approximately half of the eleven CBF orthologs at FR-H2 are duplicated in individual genomes. One of these duplications discriminates vrn-H1 genotypes from Vrn-H1 genotypes. The vrn-H1 winter allele genotypes harbor tandem segmental duplications through the CBF2A-CBF4B genomic region and maintain two distinct CBF2 paralogs, while the Vrn-H1 spring allele genotypes harbor single copies of CBF2 and CBF4. An additional CBF gene, CBF13, is a pseudogene interrupted by multiple non-sense codons in 'Tremois' whereas CBF13 is a complete uninterrupted coding sequence in 'Dicktoo' and 'Nure'. DNA blot hybridization with wheat DNAs reveals greater copy numbers of CBF14 also occurs in winter wheats than in spring wheats. These data indicate that variation in CBF gene copy numbers is widespread in the Triticeae and suggest selection for winter hardiness co-selects winter alleles at both VRN-1 and FR-2.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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