GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001763
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
scent
Trait State in Taxon B
no scent
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Mimulus lewisii; Erythranthe lewisii (Pursh) G.L.Nesom & N.S.Fraga; Mimulus lewisii Pursh
Rank
species
Lineage
Show more ... Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Phrymaceae; Erythranthe
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Mimulus bicolor; Erythranthe bicolor (Hartw. ex Benth.) G.L.Nesom & N.S.Fraga; Mimulus bicolor Hartw. ex Benth.
Rank
species
Lineage
Show more ... Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Phrymaceae; Erythranthe
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
TPS02
Synonyms
-
String
-
Sequence Similarities
Belongs to the terpene synthase family. Tpsb subfamily.
UniProtKB
Arabidopsis thaliana
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Insertion Size
1-9 bp
Molecular Details of the Mutation
2-bp insertion in the second exon, producing a frameshift mutation and premature termination
Experimental Evidence
Authors
Peng F; Byers KJRP; Bradshaw HD
Abstract
Pollinator-mediated selection on flower phenotypes (e.g., shape, color, scent) is key to understanding the adaptive radiation of angiosperms, many of which have evolved specialized relationships with a particular guild of animal pollinators (e.g., birds, bats, moths, bees). E-β-Ocimene, a monoterpene produced by OCIMENE SYNTHASE (OS) in Mimulus lewisii, is a floral scent important in attracting the species' bumblebee pollinators. The taxa closely related to M. lewisii have evolved several different pollination syndromes, including hummingbird pollination and self pollination (autogamy). We are interested in how floral scent variation contributed to species diversification in this clade.

We analyzed variation in E-β-ocimene emission within this Mimulus clade and explored its molecular basis through a combination of DNA sequencing, reverse transcriptase PCR, and enzyme functional analysis in vitro.

We found that none of the taxa, other than M. lewisii, emitted E-β-ocimene from flowers. But the molecular basis underlying loss of E-β-ocimene emission is unique in each taxon, including deletion, missense, or frameshift mutations in the OS gene, and potential posttranscriptional downregulation.

The molecular evidence suggests that parallel loss-of-function in OS is the best explanation for the observed pattern of E-β-ocimene emission, likely as the result of natural selection.

© 2017 Botanical Society of America.
Additional References
RELATED GEPHE
Related Haplotypes
2
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