GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00001787
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
sensitive
Trait State in Taxon B
resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Aspergillus nidulellus; ATCC 10074; ATCC:10074; A. nidulans; Aspergilus nidulans
Rank
species
Lineage
cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Aspergillus nidulellus; ATCC 10074; ATCC:10074; A. nidulans; Aspergilus nidulans
Rank
species
Lineage
cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Phe200Tyr
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Phe Tyr 200
Authors
Jung MK; Wilder IB; Oakley BR
Abstract
We report the cloning and sequencing of 18 mutant alleles of the benA, beta-tubulin gene of Aspergillus nidulans that confer resistance to the benzimidazole antifungal, antimicrotubule compounds benomyl, carbendazim, nocodazole, and thiabendazole. In 12 cases, amino acid 6 was changed from histidine to tyrosine or leucine. In four cases, amino acid 198 was changed from glutamic acid to aspartic acid, glutamine, or lysine. In two cases, amino acid 200 was altered from phenylalanine to tyrosine. These data, along with previous data indicating that amino acid 165 is involved in the binding of the R2 group of these compounds [Jung and Oakley, 1990: Cell Motil. Cytoskeleton 17:87-94], suggest that regions of beta-tubulin containing amino acids 6, 165, and 198-200 interact to form the binding site of benzimidazole antimicrotubule agents. These results also suggest that the presence of phenylalanine at amino acid 200 contributes to the great sensitivity of many fungi to benzimidazole antimicrotubule agents.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
COMMENTS
Effect of the amino acid change tested in C. elegans - UniProtKB in Aspergillus is P10653 @&
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