GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00001859
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Cimex lectularius
Trait State in Taxon B
Cimex lectularius - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
bed bug
Synonyms
bed bug; Cimex lectularius Linnaeus, 1758; Cimex lectularis
Rank
species
Lineage
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acea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Prosorrhyncha; Heteroptera; Euheteroptera; Neoheteroptera; Panheteroptera; Cimicomorpha; Cimicoidea; Cimicidae; Cimex
Parent
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
bed bug
Synonyms
bed bug; Cimex lectularius Linnaeus, 1758; Cimex lectularis
Rank
species
Lineage
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acea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Prosorrhyncha; Heteroptera; Euheteroptera; Neoheteroptera; Panheteroptera; Cimicomorpha; Cimicoidea; Cimicidae; Cimex
Parent
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
para
Synonyms
bas; bss; CG9907; Dmel\CG9907; DmNav; DmNav1; DmNa[[v]]; DmNa[[V]]; DmNa[[v]]1; l(1)14Da; l(1)ESHS48; lincRNA.S9469; Nav1; Ocd; olfD; par; sbl; sbl-1; Shu; Shudderer
String
Sequence Similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Para subfamily.
GO - Molecular Function
GO:0005509 : calcium ion binding
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GO - Biological Process
GO - Cellular Component
GO:0005887 : integral component of plasma membrane
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Mutation #1
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
L925I
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | CTT | ATT | - |
Amino-acid | Leu | Ile | 925 |
Main Reference
Authors
Yoon KS; Kwon DH; Strycharz JP; Hollingsworth CS; Lee SH; Clark JM
Abstract
This study establishes deltamethrin resistance in a common bed bug, Cimex lectularius L., population collected from New York City (NY-BB). The NY-BB population was 264-fold more resistant to 1% deltamethrin in contact bioassay compared with an insecticide-susceptible population collected in Florida (FL-BB). General esterase, glutathione S-transferase, and 7-ethoxycoumarin O-deethylase activities of NY-BB were not statistically different from those of FL-BB. cDNA fragments that encoded the open reading frame of voltage-sensitive sodium channel alpha-subunit genes from the FL-BB and NY-BB populations, respectively, were obtained by homology probing polymerase chain reaction (PCR) and sequenced. Sequence alignment of the internal and 5' and 3' rapid amplification of cDNA ends (RACE) fragments generated a 6500-bp cDNA sequence contig, which was composed of a 6084-bp open reading frame (ORF) encoding 2027 amino acid residues and 186-bp 5' and 230-bp 3' untranslated regions (5' and 3' UTRs, respectively). Sequence comparisons of the open reading frames of the alpha-subunit genes identified two point mutations (V419L and L925I) that were presented only in the NY-BB population. L925I, located the intracellular loop between IIS4 and IIS5, has been previously found in a highly pyrethroid-resistant populations of whitefly (Bemisia tabaci). V419L, located in the IS6 transmembrane segment, is a novel mutation. A Val to Met mutation at the corresponding position of the bed bug V419, however, has been identified in the tobacco budworm as a kdr-type mutation. This evidence suggests that the two mutations are likely the major resistance-causing mutations in the deltamethrin-resistant NY-BB through a knockdown-type nerve insensitivity mechanism.
Mutation #2
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
V419M - a Val to Met mutation at the corresponding position of the bed bug V419 has been identified in the tobacco budworm Heliothis virescens (Park et al. 1997) and functionally validated as a kdr-type mutation (Lee and Soderlund 2001)
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | GTC | CTC | - |
Amino-acid | Val | Met | 419 |
Main Reference
Authors
Yoon KS; Kwon DH; Strycharz JP; Hollingsworth CS; Lee SH; Clark JM
Abstract
This study establishes deltamethrin resistance in a common bed bug, Cimex lectularius L., population collected from New York City (NY-BB). The NY-BB population was 264-fold more resistant to 1% deltamethrin in contact bioassay compared with an insecticide-susceptible population collected in Florida (FL-BB). General esterase, glutathione S-transferase, and 7-ethoxycoumarin O-deethylase activities of NY-BB were not statistically different from those of FL-BB. cDNA fragments that encoded the open reading frame of voltage-sensitive sodium channel alpha-subunit genes from the FL-BB and NY-BB populations, respectively, were obtained by homology probing polymerase chain reaction (PCR) and sequenced. Sequence alignment of the internal and 5' and 3' rapid amplification of cDNA ends (RACE) fragments generated a 6500-bp cDNA sequence contig, which was composed of a 6084-bp open reading frame (ORF) encoding 2027 amino acid residues and 186-bp 5' and 230-bp 3' untranslated regions (5' and 3' UTRs, respectively). Sequence comparisons of the open reading frames of the alpha-subunit genes identified two point mutations (V419L and L925I) that were presented only in the NY-BB population. L925I, located the intracellular loop between IIS4 and IIS5, has been previously found in a highly pyrethroid-resistant populations of whitefly (Bemisia tabaci). V419L, located in the IS6 transmembrane segment, is a novel mutation. A Val to Met mutation at the corresponding position of the bed bug V419, however, has been identified in the tobacco budworm as a kdr-type mutation. This evidence suggests that the two mutations are likely the major resistance-causing mutations in the deltamethrin-resistant NY-BB through a knockdown-type nerve insensitivity mechanism.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
@SeveralMutationsWithEffect The other species name used in publications is: Cimex luctularis.
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