GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001860
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Varroa destructor -sensitive to pyrethroids
Trait State in Taxon B
Varroa destructor - resistant to pyrethroids
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
honeybee mite
Synonyms
honeybee mite; honeybee ectoparasitic mite; Varroa destructor Anderson & Trueman, 2000
Rank
species
Lineage
Show more ... etazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Parasitiformes; Mesostigmata; Monogynaspida; Gamasina; Dermanyssoidea; Varroidae; Varroa
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
honeybee mite
Synonyms
honeybee mite; honeybee ectoparasitic mite; Varroa destructor Anderson & Trueman, 2000
Rank
species
Lineage
Show more ... etazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Parasitiformes; Mesostigmata; Monogynaspida; Gamasina; Dermanyssoidea; Varroidae; Varroa
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Mutation #1
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
L925M
Experimental Evidence
Taxon A Taxon B Position
Codon CTG ATG -
Amino-acid Leu Met 925
Authors
González-Cabrera J; Rodríguez-Vargas S; Davies TG; Field LM; Schmehl D; Ellis JD; Krieger K; Williamson MS
Abstract
The parasitic mite Varroa destructor has a significant worldwide impact on bee colony health. In the absence of control measures, parasitized colonies invariably collapse within 3 years. The synthetic pyrethroids tau-fluvalinate and flumethrin have proven very effective at managing this mite within apiaries, but intensive control programs based mainly on one active ingredient have led to many reports of pyrethroid resistance. In Europe, a modification of leucine to valine at position 925 (L925V) of the V. destructor voltage-gated sodium channel was correlated with resistance, the mutation being found at high frequency exclusively in hives with a recent history of pyrethroid treatment. Here, we identify two novel mutations, L925M and L925I, in tau-fluvalinate resistant V. destructor collected at seven sites across Florida and Georgia in the Southeastern region of the USA. Using a multiplexed TaqMan® allelic discrimination assay, these mutations were found to be present in 98% of the mites surviving tau-fluvalinate treatment. The mutations were also found in 45% of the non-treated mites, suggesting a high potential for resistance evolution if selection pressure is applied. The results from a more extensive monitoring programme, using the Taqman® assay described here, would clearly help beekeepers with their decision making as to when to include or exclude pyrethroid control products and thereby facilitate more effective mite management programmes.
Additional References
Mutation #2
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
L925I
Experimental Evidence
Taxon A Taxon B Position
Codon CTG ATA -
Amino-acid Leu Ile 925
Authors
González-Cabrera J; Rodríguez-Vargas S; Davies TG; Field LM; Schmehl D; Ellis JD; Krieger K; Williamson MS
Abstract
The parasitic mite Varroa destructor has a significant worldwide impact on bee colony health. In the absence of control measures, parasitized colonies invariably collapse within 3 years. The synthetic pyrethroids tau-fluvalinate and flumethrin have proven very effective at managing this mite within apiaries, but intensive control programs based mainly on one active ingredient have led to many reports of pyrethroid resistance. In Europe, a modification of leucine to valine at position 925 (L925V) of the V. destructor voltage-gated sodium channel was correlated with resistance, the mutation being found at high frequency exclusively in hives with a recent history of pyrethroid treatment. Here, we identify two novel mutations, L925M and L925I, in tau-fluvalinate resistant V. destructor collected at seven sites across Florida and Georgia in the Southeastern region of the USA. Using a multiplexed TaqMan® allelic discrimination assay, these mutations were found to be present in 98% of the mites surviving tau-fluvalinate treatment. The mutations were also found in 45% of the non-treated mites, suggesting a high potential for resistance evolution if selection pressure is applied. The results from a more extensive monitoring programme, using the Taqman® assay described here, would clearly help beekeepers with their decision making as to when to include or exclude pyrethroid control products and thereby facilitate more effective mite management programmes.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
@TwoNucleotideChangesInSameCodon or @SuccessiveMutationsAtSameCodon The codons found at position 925 are: CTG, ATG, ATA
YOUR FEEDBACK is welcome!