GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00001888
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Eleusine indica - sensitive
Trait State in Taxon B
Eleusine indica - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
goosegrass
Synonyms
goosegrass; crabgrass; fowl foot grass; wire grass; yardgrass; Eleusine indica (L.) Gaertn.
Rank
species
Lineage
Show more ... ta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Cynodonteae; Eleusininae; Eleusine
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
goosegrass
Synonyms
goosegrass; crabgrass; fowl foot grass; wire grass; yardgrass; Eleusine indica (L.) Gaertn.
Rank
species
Lineage
Show more ... ta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Cynodonteae; Eleusininae; Eleusine
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
UniProtKB
Arabidopsis thaliana
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Pro106Ser leading to 2-4-fold resistance
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Pro Ser 106
Authors
Baerson SR; Rodriguez DJ; Tran M; Feng Y; Biest NA; Dill GM
Abstract
The spontaneous occurrence of resistance to the herbicide glyphosate in weed species has been an extremely infrequent event, despite over 20 years of extensive use. Recently, a glyphosate-resistant biotype of goosegrass (Eleusine indica) was identified in Malaysia exhibiting an LD(50) value approximately 2- to 4-fold greater than the sensitive biotype collected from the same region. A comparison of the inhibition of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity by glyphosate in extracts prepared from the resistant (R) and sensitive (S) biotypes revealed an approximately 5-fold higher IC(50)(glyphosate) for the (R) biotype. Sequence comparisons of the predicted EPSPS mature protein coding regions from both biotypes revealed four single-nucleotide differences, two of which result in amino acid changes. One of these changes, a proline to serine substitution at position 106 in the (R) biotype, corresponds to a substitution previously identified in a glyphosate-insensitive EPSPS enzyme from Salmonella typhimurium. Kinetic data generated for the recombinant enzymes suggests that the second substitution identified in the (R) EPSPS does not contribute significantly to its reduced glyphosate sensitivity. Escherichia coli aroA- (EPSPS deficient) strains expressing the mature EPSPS enzyme from the (R) biotype exhibited an approximately 3-fold increase in glyphosate tolerance relative to strains expressing the mature EPSPS from the (S) biotype. These results provide the first evidence for an altered EPSPS enzyme as an underlying component of evolved glyphosate resistance in any plant species.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
1
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