GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00001980
Main curator
Courtier
PHENOTYPIC CHANGE
Trait #1
Trait Category
Trait
Trait State in Taxon A
other Drosophila simulans
Trait State in Taxon B
Drosophila simulans homozygous for the deletion - reduction in cox activity - compensated by an increase in mtDNA copy number and ADP:O ratio that is correlated with higher early fertility - reduced physical activity in homozygotes on day 18 and reduced mean survival
Trait #2
Trait Category
Trait
Trait State in Taxon A
Drosophila simulans
Trait State in Taxon B
Drosophila simulans - reduction in cox activity - compensated by an increase in mtDNA copy number and ADP:O ratio that is correlated with higher early fertility - reduced physical activity in homozygotes on day 18 and reduced mean survival
Trait #3
Trait Category
Trait
Trait State in Taxon A
Drosophila simulans
Trait State in Taxon B
Drosophila simulans - reduction in cox activity - compensated by an increase in mtDNA copy number and ADP:O ratio that is correlated with higher early fertility - reduced physical activity in homozygotes on day 18 and reduced mean survival
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
-
Rank
species
Lineage
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Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
-
Rank
species
Lineage
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Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
COX7A
Synonyms
CG9603; Cox7a; COXJ; COXK; Dmel\CG9603; VIIa
String
Sequence Similarities
Belongs to the cytochrome c oxidase VIIa family.
GO - Molecular Function
GO - Biological Process
GO - Cellular Component
GO:0005739 : mitochondrion
... show more
UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
Deletion Size
1-9 bp
Molecular Details of the Mutation
Deletion of two amino acids (Trp85 and Val86). The deletion occurs in subunit 7A of the mitochondrial electron trans-port chain protein cytochrome c oxidase (cox7A). The nuclear encoded cox7A gene produces a protein that isimported into the mitochondrion and forms a subunit of complexIV (cytochrome c oxidase) of the electron transport chain.
Experimental Evidence
Authors
Melvin RG; Katewa SD; Ballard JW
Abstract
A problem with studying evolutionary dynamics of mitochondrial (mt) DNA is that classical population genetic techniques cannot identify selected substitutions because of genetic hitchhiking. We circumvented this problem by employing a candidate complex approach to study sequence variation in cytochrome c oxidase (COX) genes within and among three distinct Drosophila simulans mtDNA haplogroups. First, we determined sequence variation in complete coding regions for all COX mtDNA and nuclear loci and their isoforms. Second, we constructed a quaternary structure model of D. simulans COX. Third, we predicted that six of nine amino acid changes in D. simulans mtDNA are likely to be functionally important. Of these seven, genetic crosses can experimentally determine the functional significance of three. Fourth, we identified two single amino acid changes and a deletion of two consecutive amino acids in nuclear encoded COX loci that are likely to influence cytochrome c oxidase activity. These data show that linking population genetics and quaternary structure modeling can lead to functional predictions of specific mtDNA amino acid mutations and validate the candidate complex approach.
RELATED GEPHE
Related Genes
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
http://flybase.org/reports/FBal0277773
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