GEPHE SUMMARY Print
Entry Status
Published
GepheID
GP00002014
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Leptinotarsa decemlineata - sensitive
Trait State in Taxon B
Leptinotarsa decemlineata - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
Colorado potato beetle
Synonyms
Leptinotarsa decimlineata; Stilodes decemlineata; Colorado potato beetle; Leptinotarsa decemlineata (Say, 1824)
Rank
species
Lineage
Show more ... andibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Coleoptera; Polyphaga; Cucujiformia; Chrysomeloidea; Chrysomelidae; Chrysomelinae; Doryphorini; Leptinotarsa
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
Colorado potato beetle
Synonyms
Leptinotarsa decimlineata; Stilodes decemlineata; Colorado potato beetle; Leptinotarsa decemlineata (Say, 1824)
Rank
species
Lineage
Show more ... andibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Coleoptera; Polyphaga; Cucujiformia; Chrysomeloidea; Chrysomelidae; Chrysomelinae; Doryphorini; Leptinotarsa
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
UniProtKB
Drosophila melanogaster
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Ser>Gly (238 is the corresponding position in Torpedo)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ser Gly 238
Authors
Zhu KY; Lee SH; Clark JM
Abstract
A serine to glycine point mutation of acetylcholinesterase (AChE, EC 1.1.1.7) was identified in an azinphosmethyl-resistant strain of Colorado potato beetle [Leptinotarsa decemlineata (Say)]. The position of the mutation corresponds to Val 238 of the Torpedo AChE and represents the first amino acid residue to form the alpha-helix, alpha-E'1. The predicted secondary structure of the mutation-containing region of AChE suggested that the transition from the turn to the alpha-helix occurs sooner in the sequence when serine is replaced by glycine. Thus, conformational changes in the AChE due to the alpha-helix deformation were expected to impinge upon both the catalytic and the peripheral binding sites, resulting in the modification of the bindings of organophosphorus insecticides and other ligands to these sites. The mutation appeared to be associated with the fitness of the beetle. The intrinsic rate of increase of the azinphosmethyl-resistant (AZ-R) strain was relatively low when the beetles were reared on the Russet Burbank potato cultivar, but was relatively high when they were reared on the NDA 1725-1 potato cultivar. Because these two potato cultivars contain different amounts of steroidal glycoalkaloids (e.g., alpha-solanine and alpha-chaconine), the different fitness of the AZ-R strain on different potato cultivars may be partially attributed to the increased sensitivity of the azinphosmethyl-resistant form of AChE to the inhibition by alpha-solanine and reduced sensitivity to alpha-chaconine as previously reported.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
1
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