GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000204
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Depressaria pastinacella
Trait State in Taxon B
Depressaria pastinacella
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Common Name
-
Synonyms
-
Rank
species
Lineage
Show more ... exapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Gelechioidea; Elachistidae; Depressariinae; Depressaria
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
-
Rank
species
Lineage
Show more ... exapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Gelechioidea; Elachistidae; Depressariinae; Depressaria
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
CYP6AB3
Synonyms
-
String
-
Sequence Similarities
Belongs to the cytochrome P450 family.
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
Depressaria pastinacella
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Ala92Val (and potentiallly 4 other a.a. substitutions)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ala Val 92
Authors
Mao W; Rupasinghe SG; Zangerl AR; Berenbaum MR; Schuler MA
Abstract
CYP6AB3v1, a cytochrome P450 monooxygenase in Depressaria pastinacella (parsnip webworm), is highly specialized for metabolizing imperatorin, a toxic furanocoumarin in the apiaceous host plants of this insect. Cloning and heterologous expression of CYP6AB3v2, an allelic variant identified in D. pastinacella, reveals that it metabolizes imperatorin at a rate (V(max) of 10.02 pmol/min/pmol of cytochrome P450 monooxygenase (P450)) significantly higher than CYP6AB3v1 (V(max) of 2.41 pmol/min/pmol) when supplemented with even low levels of cytochrome P450 reductase. Comparisons of the NADPH consumption rates for these variants indicate that CYP6AB3v2 utilizes this electron source at a faster rate than does CYP6AB3v1. Molecular modeling of the five amino acid differences between these variants and their potential interactions with P450 reductase suggests that replacement of Val(92) on the proximal face of CYP6AB3v1 with Ala(92) in CYP6AB3v2 affects interactions with P450 reductase so as to enhance its catalytic activity. Allelic variation at this locus potentially allows D. pastinacella to adapt to both intraspecific and interspecific variation in imperatorin concentrations in its host plants.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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