GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002041
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
sensitive
Trait State in Taxon B
resistant - clade I
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
-
Rank
species
Lineage
cellular organisms; Eukaryota; Stramenopiles; Oomycetes; Peronosporales; Peronosporaceae; Plasmopara
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
-
Rank
species
Lineage
cellular organisms; Eukaryota; Stramenopiles; Oomycetes; Peronosporales; Peronosporaceae; Plasmopara
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Gly143Ala G1256C
Experimental Evidence
Taxon A Taxon B Position
Codon GGN GCN 1256
Amino-acid Gly Ala 143
Authors
Chen WJ; Delmotte F; Richard-Cervera S; Douence L; Greif C; Corio-Costet MF
Abstract
Quinone outside inhibiting (QoI) fungicides represent one of the most widely used groups of fungicides used to control agriculturally important fungal pathogens. They inhibit the cytochrome bc1 complex of mitochondrial respiration. Soon after their introduction onto the market in 1996, QoI fungicide-resistant isolates were detected in field plant pathogen populations of a large range of species. However, there is still little understanding of the processes driving the development of QoI fungicide resistance in plant pathogens. In particular, it is unknown whether fungicide resistance occurs independently in isolated populations or if it appears once and then spreads globally by migration. Here, we provide the first case study of the evolutionary processes that lead to the emergence of QoI fungicide resistance in the plant pathogen Plasmopara viticola. Sequence analysis of the complete cytochrome b gene showed that all resistant isolates carried a mutation resulting in the replacement of glycine by alanine at codon 143 (G143A). Phylogenetic analysis of a large mitochondrial DNA fragment including the cytochrome b gene (2,281 bp) across a wide range of European P. viticola isolates allowed the detection of four major haplotypes belonging to two distinct clades, each of which contains a different QoI fungicide resistance allele. This is the first demonstration that a selected substitution conferring resistance to a fungicide has occurred several times in a plant-pathogen system. Finally, a high population structure was found when the frequency of QoI fungicide resistance haplotypes was assessed in 17 French vineyards, indicating that pathogen populations might be under strong directional selection for local adaptation to fungicide pressure.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
1
COMMENTS
@MitochondrialGene @Parallelism - phylogenetic reconstruction shows that the same mutation G>C Gly>Ala occurred independently in two different haplotypes
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