GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002043
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
sensitive
Trait State in Taxon B
resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
Chlamydomonas smithii; Chlamydomonas reinhardtii P.A.Dangeard; Chlamydomonas smithii R.W.Howshaw & H.Ettl; SAG 53.72; SAG 54.72; SAG:53.72; SAG:54.72; UTEX 90; UTEX:90; Chlamydomonas reihhardtii; Chlamydonas reinhardtii
Rank
species
Lineage
cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
Chlamydomonas smithii; Chlamydomonas reinhardtii P.A.Dangeard; Chlamydomonas smithii R.W.Howshaw & H.Ettl; SAG 53.72; SAG 54.72; SAG:53.72; SAG:54.72; UTEX 90; UTEX:90; Chlamydomonas reihhardtii; Chlamydonas reinhardtii
Rank
species
Lineage
cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
UQCRFS1
Synonyms
RIP1; RIS1; RISP; UQCR5
String
Sequence Similarities
-
GO - Molecular Function
GO:0046872 : metal ion binding
... show more
GO - Biological Process
GO - Cellular Component
GO:0016021 : integral component of membrane
... show more
UniProtKB
Homo sapiens
Homo sapiens
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
F129L
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | Phe | Lys | 129 |
Main Reference
Authors
Bennoun P; Delosme M; Kück U
Abstract
We describe the genetic and molecular analysis of the first non-Mendelian mutants of Chlamydomonas reinhardtii resistant to myxothiazol, an inhibitor of the respiratory cytochrome bc1 complex. Using a set of seven oligonucleotide probes, restriction fragments containing the mitochondrial cytochrome b (cyt b) gene from C. reinhardtii were isolated from a mitochondrial DNA library. This gene is located adjacent to the gene for subunit 4 of the mitochondrial NADH-dehydrogenase (ND4), near one end of the 15.8-kb linear mitochondrial genome of C. reinhardtii. The algal cytochrome b apoprotein contains 381 amino-acid residues and exhibits a sequence similarity of about 59% with other plant cytochrome b proteins. The cyt b gene from four myxothiazol resistant mutants of C. reinhardtii was amplified for DNA sequence analysis. In comparison to the wild-type strain, all mutants contain an identical point mutation in the cyt b gene, leading to a change of a phenylalanine codon to a leucine codon at amino acid position 129 of the cytochrome b protein. Segregation analysis in tetrads from reciprocal crosses of mutants with wild type shows a strict uniparental inheritance of this mutation from the mating type minus parent (UP-). However, mitochondrial markers from both parents are recovered in vegetative diploids in variable proportions from one experiment to the next for a given cross. On the average, a strong bias is seen for markers inherited from the mating type minus parent.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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