GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002043
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
sensitive
Trait State in Taxon B
resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Chlamydomonas smithii; Chlamydomonas reinhardtii P.A.Dangeard; Chlamydomonas smithii R.W.Howshaw & H.Ettl; SAG 53.72; SAG 54.72; SAG:53.72; SAG:54.72; UTEX 90; UTEX:90; Chlamydomonas reihhardtii; Chlamydonas reinhardtii
Rank
species
Lineage
cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Chlamydomonas smithii; Chlamydomonas reinhardtii P.A.Dangeard; Chlamydomonas smithii R.W.Howshaw & H.Ettl; SAG 53.72; SAG 54.72; SAG:53.72; SAG:54.72; UTEX 90; UTEX:90; Chlamydomonas reihhardtii; Chlamydonas reinhardtii
Rank
species
Lineage
cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
F129L
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Phe Lys 129
Authors
Bennoun P; Delosme M; Kück U
Abstract
We describe the genetic and molecular analysis of the first non-Mendelian mutants of Chlamydomonas reinhardtii resistant to myxothiazol, an inhibitor of the respiratory cytochrome bc1 complex. Using a set of seven oligonucleotide probes, restriction fragments containing the mitochondrial cytochrome b (cyt b) gene from C. reinhardtii were isolated from a mitochondrial DNA library. This gene is located adjacent to the gene for subunit 4 of the mitochondrial NADH-dehydrogenase (ND4), near one end of the 15.8-kb linear mitochondrial genome of C. reinhardtii. The algal cytochrome b apoprotein contains 381 amino-acid residues and exhibits a sequence similarity of about 59% with other plant cytochrome b proteins. The cyt b gene from four myxothiazol resistant mutants of C. reinhardtii was amplified for DNA sequence analysis. In comparison to the wild-type strain, all mutants contain an identical point mutation in the cyt b gene, leading to a change of a phenylalanine codon to a leucine codon at amino acid position 129 of the cytochrome b protein. Segregation analysis in tetrads from reciprocal crosses of mutants with wild type shows a strict uniparental inheritance of this mutation from the mating type minus parent (UP-). However, mitochondrial markers from both parents are recovered in vegetative diploids in variable proportions from one experiment to the next for a given cross. On the average, a strong bias is seen for markers inherited from the mating type minus parent.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
COMMENTS
@MitochondrialGene
YOUR FEEDBACK is welcome!