GEPHE SUMMARY Print
Trait State in Taxon A
Trait State in Taxon B
Saccharomyces capensis; Saccharomyces italicus; Saccharomyces oviformis; Saccharomyces uvarum var. melibiosus; baker's yeast; S. cerevisiae; brewer's yeast; ATCC 18824; ATCC:18824; CBS 1171; CBS:1171; NRRL Y-12632; NRRL:Y:12632; Saccaromyces cerevisiae; Saccharomyce cerevisiae; Saccharomyes cerevisiae; Sccharomyces cerevisiae
cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Schizosaccharomyces malidevorans; fission yeast; BCRC:21461; CBS 356; CBS:356; CCRC 21461; CCRC:21461; DBVPG 6277; DBVPG:6277; JCM 8274; JCM:8274; MUCL 30245; MUCL:30245; NRRL Y-12796; NRRL:Y:12796; Schizosaccharomyces pombeP
cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Generic Gene Name
RIP1; RIS1; RISP; UQCR5
GO - Molecular Function
GO:0046872 : metal ion binding ... show more
GO - Biological Process
GO - Cellular Component
GO:0016021 : integral component of membrane ... show more
SNP Coding Change
Molecular Details of the Mutation
|Taxon A||Taxon B||Position|
Kraiczy P; Haase U; Gencic S; Flindt S; Anke T; Brandt U; Von Jagow G
Mitochondria from the strobilurin A producing basidiomycetes Strobilurus tenacellus and Mycena galopoda exhibit natural resistance to (E)-beta-methoxyacrylate inhibitors of the ubiquinol oxidation center(center Qp) of the cytochrome bc1 complex. Isolated cytochrome bc1 complex from S. tenacellus was found to be highly similar to that of Saccharomyces cerevisiae with respect to subunit composition, as well as spectral characteristics and midpoint potentials of the heme centers. To understand the molecular basis of natural resistance, we determined the exon/intron organization and deduced the sequences of cytochromes b from S. tenacellus, M. galopoda and a third basidiomycete, Mycena viridimarginata, which produces no strobilurin A. Comparative sequence analysis of two regions of cytochrome b known to contribute to the formation of center Qp suggested that the generally lower sensitivity of all three basidiomycetes was due to the replacement of a small amino acid residue in position 127 by isoleucine. For M. galopoda replacement of Gly143 by alanine and Gly153 by serine, for S. tenacellus replacement of a small residue in position 254 by glutamine and Asn261 by aspartate was found to be the likely causes for resistance to (E)-beta-methoxyacrylates. The latter exchange is also found in Schizosaccharomyces pombe, which we found also to be naturally resistant to (E)-beta-methoxyacrylates.
@MitochondrialGene - The Asn261Asp mutation has been found in other resistant taxa. Not clear if it is an interspecific or intraspecific change since a single strain of S. pombe was sequenced in this study. A alignment of many cyt b sequences from S. pombe would be required to check if the change is intra versus interspecific.
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