GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000205
Main curator
Martin
PHENOTYPIC CHANGE
Trait #1
Trait Category
Trait State in Taxon A
Other butterflies
Trait State in Taxon B
Papilio polyxenes
Trait #2
Trait Category
Trait State in Taxon A
-
Trait State in Taxon B
-
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Latin Name
Common Name
brushfoots
Synonyms
brushfoots; brush-footed butterflies
Rank
family
Lineage
Show more ... Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Papilionoidea
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
black swallowtail
Synonyms
black swallowtail; American swallowtail; eastern black swallowtail; Papilio polyxenes Fabricius, 1775
Rank
species
Lineage
Show more ... ondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Papilionoidea; Papilionidae; Papilioninae; Papilionini; Papilio
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
CYP6B1
Synonyms
-
String
-
Sequence Similarities
Belongs to the cytochrome P450 family.
GO - Biological Process
-
UniProtKB
Papilio polyxenes
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Ile484Phe and probably other a.a. substitutions
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ile Phe 484
Authors
Li W; Schuler MA; Berenbaum MR
Abstract
Diversification of cytochrome P450 monooxygenases (P450s) is thought to result from antagonistic interactions between plants and their herbivorous enemies. However, little direct evidence demonstrates the relationship between selection by plant toxins and adaptive changes in herbivore P450s. Here we show that the furanocoumarin-metabolic activity of CYP6B proteins in two species of swallowtail caterpillars is associated with the probability of encountering host plant furanocoumarins. Catalytic activity was compared in two closely related CYP6B4 and CYP6B17 groups in the polyphagous congeners Papilio glaucus and Papilio canadensis. Generally, P450s from P. glaucus, which feeds occasionally on furanocoumarin-containing host plants, display higher activities against furanocoumarins than those from P. canadensis, which normally does not encounter furanocoumarins. These P450s in turn catalyze a larger range of furanocoumarins at lower efficiency than CYP6B1, a P450 from Papilio polyxenes, which feeds exclusively on furanocoumarin-containing host plants. Reconstruction of the ancestral CYP6B sequences using maximum likelihood predictions and comparisons of the sequence and geometry of their active sites to those of contemporary CYP6B proteins indicate that host plant diversity is directly related to P450 activity and inversely related to substrate specificity. These predictions suggest that, along the lineage leading to Papilio P450s, the ancestral, highly versatile CYP6B protein presumed to exist in a polyphagous species evolved through time into a more efficient and specialized CYP6B1-like protein in Papilio species with continual exposure to furanocoumarins. Further diversification of Papilio CYP6Bs has likely involved interspersed events of positive selection in oligophagous species and relaxation of functional constraints in polyphagous species.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
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