GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000206
Main curator
Martin
PHENOTYPIC CHANGE
Trait #1
Trait Category
Trait State in Taxon A
Other butterflies
Trait State in Taxon B
Papilio glaucus and Papilio canadensis
Trait #2
Trait Category
Trait State in Taxon A
-
Trait State in Taxon B
-
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Latin Name
Common Name
brushfoots
Synonyms
brushfoots; brush-footed butterflies
Rank
family
Lineage
Show more ... Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Papilionoidea
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
eastern tiger swallowtail
Synonyms
Pterourus glaucus; eastern tiger swallowtail; tiger swallowtail; Papilio glaucus Linnaeus, 1758
Rank
species
Lineage
Show more ... ondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Papilionoidea; Papilionidae; Papilioninae; Papilionini; Papilio
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
CYP6B4
Synonyms
-
String
-
Sequence Similarities
Belongs to the cytochrome P450 family.
GO - Biological Process
-
UniProtKB
Papilio glaucus
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Lys484Ser and probably other a.a. substitutions
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Lys Ser 484
Authors
Mao W; Schuler MA; Berenbaum MR
Abstract
Although substrate-specific CYP6B1 and CYP6B3 enzymes in Papilio polyxenes contribute to specialization on furanocoumarin-containing host plants, CYP6B4 and CYP6B17 enzymes in the polyphagous Papilio glaucus and Papilio canadensis have a broader range of substrates. Papilio multicaudatus, an oligophage with one furanocoumarin-containing host, is putatively ancestral to polyphagous Papilio species. Furanocoumarin-inducible CYP6B33-CYP6B37 and CYP6AB6 were characterized from this species. Heterologous expression of CYP6B33 revealed furanocoumarin metabolism resembling that of CYP6B4-CYP6B17 enzymes from P. glaucus and P. canadensis. Molecular models of CYP6B33 and CYP6B4 indicate that seven conserved aromatic side chains stabilize their hydrophobic catalytic sites and that a Lys484-Ser484 substitution enlarges the CYP6B4 active site pocket to increase the predicted distance between the substrate and reactive oxygen relative to CYP6B1. Loss of specialization in this lineage may have resulted from relatively few mutational changes, allowing acquisition of broader catalytic activities without loss of ancestral furanocoumarin-metabolizing activities.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
COMMENTS
YOUR FEEDBACK is welcome!