GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002064
Main curator
Cao
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Plutella xylostella -diamide (chlorantraniliprole) less resistant
Trait State in Taxon B
Plutella xylostella - diamide (chlorantraniliprole) more resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
resistant population in Chinesecabbage fields in Tonghai; Yunnan province (TH strain)
GENOTYPIC CHANGE
Generic Gene Name
CYP6BG1
Synonyms
-
String
-
Sequence Similarities
Belongs to the cytochrome P450 family.
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
Plutella xylostella
GenebankID or UniProtKB
Plutella xylostella
Presumptive Null
Molecular Type
Aberration Type
Molecular Details of the Mutation
promoters tested in luciferase assays
Experimental Evidence
Authors
Li X; Shan C; Li F; Liang P; Smagghe G; Gao X
Abstract
Cytochrome P450-mediated detoxification plays an important role in the development of insecticide resistance. Previous studies have demonstrated that overexpression of CYP6BG1 was responsible for permethrin resistance in Plutella xylostella, and our experiments also showed that upregulation of this gene is associated with chlorantraniliprole resistance in P. xylostella. However, the transcriptional regulation involved in the expression of CYP6BG1 remains unknown. To further investigate the regulation of CYP6BG1 expression, the promoters of this gene were cloned and analyzed from one susceptible and four different resistant populations of P. xylostella.

First, the promoter region of P. xylostella CYP6BG1 was compared in five populations, and three types of 5'-flanking region were found. Second, the region between -562 and +49 of CYP6BG1 in a field population (TH) of P. xylostella showed the highest promoter activity and could be induced by chlorantraniliprole. Third, the transcriptional factor FTZ-F1, which is an orphan nuclear receptor and binds to the fushi tarazu (ftz) gene, was predicted by the online software Alggen and Jaspar. It was proved to regulate the expression of CYP6BG1 by RNAi. The expression levels of FTZ-F1 and CYP6BG1 could be induced by chlorantraniliprole and were significantly higher in the resistant populations.

These data give a better understanding of the transcriptional regulation of an important insecticide detoxification enzyme gene, and therefore will help in understanding the molecular mechanisms of insecticide resistance in P. xylostella. © 2018 Society of Chemical Industry.

© 2018 Society of Chemical Industry.
RELATED GEPHE
Related Haplotypes
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