GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000207
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Drosophila melanogaster
Trait State in Taxon B
Drosophila melanogaster
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
fruit fly
Synonyms
Sophophora melanogaster; fruit fly; Drosophila melanogaster Meigen, 1830; Sophophora melanogaster (Meigen, 1830); Drosophila melangaster
Rank
species
Lineage
Show more ... Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
fruit fly
Synonyms
Sophophora melanogaster; fruit fly; Drosophila melanogaster Meigen, 1830; Sophophora melanogaster (Meigen, 1830); Drosophila melangaster
Rank
species
Lineage
Show more ... Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
Cyp6g1
Synonyms
6g1; anon-WO03025223.16; anon-WO03025223.17; CG8453; Cyp6-like; cyp6g1; Cyp6G1; CYP6g1; CYP6G1; Cyp6gl; DDT-R; Dmel-Cyp6g1; Dmel\CG8453; RDDT; RI; RI[DDT]; RI[II]; Rst(2)DDT; CYP6-like
Sequence Similarities
Belongs to the cytochrome P450 family.
UniProtKB
Drosophila melanogaster
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Insertion Size
1-10 kb
Molecular Details of the Mutation
Insertion of retrotransposon A 291bp upstream of the Cyp6g1 transcription start site
Experimental Evidence
Authors
Chung H; Bogwitz MR; McCart C; Andrianopoulos A; Ffrench-Constant RH; Batterham P; Daborn PJ
Abstract
Transposable elements are a major mutation source and powerful agents of adaptive change. Some transposable element insertions in genomes increase to a high frequency because of the selective advantage the mutant phenotype provides. Cyp6g1-mediated insecticide resistance in Drosophila melanogaster is due to the upregulation of the cytochrome P450 gene Cyp6g1, leading to the resistance to a variety of insecticide classes. The upregulation of Cyp6g1 is correlated with the presence of the long terminal repeat (LTR) of an Accord retrotransposon inserted 291bp upstream of the Cyp6g1 transcription start site. This resistant allele (DDT-R) is currently at a high frequency in D. melanogaster populations around the world. Here, we characterize the spatial expression of Cyp6g1 in insecticide-resistant and -susceptible strains. We show that the Accord LTR insertion is indeed the resistance-associated mutation and demonstrate that the Accord LTR carries regulatory sequences that increase the expression of Cyp6g1 in tissues important for detoxification, the midgut, Malpighian tubules, and the fat body. This study provides a significant example of how changes in tissue-specific gene expression caused by transposable-element insertions can contribute to adaptation.
Additional References
COMMENTS
@TE - http://flybase.org/reports/FBal0014805
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