GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002073
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Arachis hypogaea (Peanut) normal oleate level
Trait State in Taxon B
Arachis hypogaea (Peanut) high oleate level
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
peanut
Synonyms
peanut; goober; ground-nut; Arachis hypogaea L.; Arachis hypogea
Rank
species
Lineage
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a; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; dalbergioids sensu lato; Dalbergieae; Pterocarpus clade; Arachis
Parent
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
peanut
Synonyms
peanut; goober; ground-nut; Arachis hypogaea L.; Arachis hypogea
Rank
species
Lineage
Show more ...
a; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; dalbergioids sensu lato; Dalbergieae; Pterocarpus clade; Arachis
Parent
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
chemically induced mutant Mycogen-Flavo
GENOTYPIC CHANGE
Generic Gene Name
FAD2B
Synonyms
FAD; FAD2
String
-
Sequence Similarities
-
GO - Molecular Function
GO - Biological Process
GO - Cellular Component
UniProtKB
Arachis hypogaea
Arachis hypogaea
Presumptive Null
Molecular Type
Aberration Type
Insertion Size
100-999 bp
Molecular Details of the Mutation
insertion of 205-bp miniature inverted-repeat transposable element (MITE) called ahMITE1 at position 665 near the center of the coding region in the case of MF; which causes a frameshift
Experimental Evidence
Authors
Patel M; Jung S; Moore K; Powell G; Ainsworth C; Abbott A
Abstract
A high-oleate trait in the cultivated peanut ( Arachis hypogaea L.) was reported to rely on the allelic composition of the two homeologous, microsomal oleoyl-PC desaturase genes ( ahFAD2A or ahFAD2B). The enzyme activity of either ahFAD2A or ahFAD2B is sufficient for a normal oleate phenotype, and a significant reduction in the levels of ahFAD2B and a mutation in ahFAD2A were reported to be responsible for the high-oleate phenotype in one chemically induced mutant (M2-225) and one derived from a naturally occurring (8-2122) mutant. Here, we report an insertion of the same miniature inverted-repeat transposable element (MITE) in the ahFAD2B gene in another chemically induced mutant (Mycogen-Flavo) and the previously characterized M2-225 mutant. In both cases, this MITE insertion in ahFAD2B causes a frameshift, resulting in a putatively truncated protein sequence in both mutants. The insertion of this MITE in ahFAD2B, in addition to the point mutation in ahFAD2A, appears to be the cause of the high-oleate phenotype in Mycogen-Flavo and M2-225 mutants. Utilizing sequences of the MITE, we developed a DNA marker strategy to differentiate the two insertion-containing mutants from the normal oleate peanut variety (AT-108) and the naturally occurring, high-oleate mutant 8-2122. Reverse transcript-PCR/differential digestion results reveal the expression of both ahFAD2A and ahFAD2B genes in Mycogen-Flavo mutant. This result is in contrast to the observation that ahFAD2B transcripts are greatly reduced in the M2-225 mutant having the MITE insertion further 3' in ahFAD2B gene.
Additional References
EXTERNAL LINKS
COMMENTS
@TE - The null mutation in FAD2A is also necessary for the high oleate level phenotype.
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