GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002091
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Ipomoea tricolor - bright blue flowers and dark-brown seeds
Trait State in Taxon B
Ipomoea tricolor - pale blue flowers with a few fine blue spots and ivory seeds with tiny dark‐brown spots
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Ipomoea rubro-caerulea; Ipomoea rubrocaerulea; Pharbitis rubro-caerulea; Pharbitis rubrocaeruleus; campanilla; flying-saucers; pearly-gates; yaxcelil; Ipomoea rubrocaerulea Hook., 1834; Ipomoea tricolor Cav., 1795; Pharbitis rubrocaeruleus (Hook.) Planch., 1854
Rank
species
Lineage
Show more ... ina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Ipomoea rubro-caerulea; Ipomoea rubrocaerulea; Pharbitis rubro-caerulea; Pharbitis rubrocaeruleus; campanilla; flying-saucers; pearly-gates; yaxcelil; Ipomoea rubrocaerulea Hook., 1834; Ipomoea tricolor Cav., 1795; Pharbitis rubrocaeruleus (Hook.) Planch., 1854
Rank
species
Lineage
Show more ... ina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
Yes
Molecular Type
Aberration Type
Insertion Size
1-10 kb
Molecular Details of the Mutation
intragenic tandem duplication of 3.3 kbp of the bHLH2 gene. Each of the tandem repeats is flanked by a 3-bp sequence AAT; indicating that the 3-bp microhomology is used to generate the tandem duplication. The transcripts in the pale-blue flower buds of the mutant contain an internal 583-bp tandem duplication that results in the production of a truncated polypeptide lacking the bHLH domain. The mRNA accumulation of most of the structural genes encoding enzymes for anthocyanin biosynthesis in the flower buds of the mutant was significantly reduced. The transcripts identical to the wild-type mRNAs for the transcriptional activator were present abundantly in blue spots of the variegated flowers whereas the transcripts containing the 583-bp tandem duplication were predominant in the pale-blue background of the same flowers. The flower and seed variegations studied here are likely to be caused by somatic homologous recombination between an intragenic tandem duplication in the gene encoding a bHLH transcriptional activator for anthocyanin biosynthesis.
Experimental Evidence
Authors
Park KI; Choi JD; Hoshino A; Morita Y; Iida S
Abstract
While the wild-type morning glory (Ipomoea tricolor) displays bright-blue flowers and dark-brown seeds, its spontaneous mutant, Blue Star, carrying the mutable ivory seed-variegated (ivs-v) allele, exhibits pale-blue flowers with a few fine blue spots and ivory seeds with tiny dark-brown spots. The mutable allele is caused by an intragenic tandem duplication of 3.3 kbp within a gene for transcriptional activator containing a basic helix-loop-helix (bHLH) DNA-binding motif. Each of the tandem repeats is flanked by a 3-bp sequence AAT, indicating that the 3-bp microhomology is used to generate the tandem duplication. The transcripts in the pale-blue flower buds of the mutant contain an internal 583-bp tandem duplication that results in the production of a truncated polypeptide lacking the bHLH domain. The mRNA accumulation of most of the structural genes encoding enzymes for anthocyanin biosynthesis in the flower buds of the mutant was significantly reduced. The transcripts identical to the wild-type mRNAs for the transcriptional activator were present abundantly in blue spots of the variegated flowers, whereas the transcripts containing the 583-bp tandem duplication were predominant in the pale-blue background of the same flowers. The flower and seed variegations studied here are likely to be caused by somatic homologous recombination between an intragenic tandem duplication in the gene encoding a bHLH transcriptional activator for anthocyanin biosynthesis, whereas various flower variegations are reported to be caused by excision of DNA transposons inserted into pigmentation genes.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
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