GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002093
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Petunia axillaris
Trait State in Taxon B
Petunia axillaris - white flowers with red and pink revertant spots
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
large white petunia; white moon petunia; Petunia axillaris (Lam.) Britton, Stern & Poggenb.; Petunia axilliaris
Rank
species
Lineage
Show more ... tina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
large white petunia; white moon petunia; Petunia axillaris (Lam.) Britton, Stern & Poggenb.; Petunia axilliaris
Rank
species
Lineage
Show more ... tina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
petunia line W138
GENOTYPIC CHANGE
Generic Gene Name
InWDR1
Synonyms
InWDR1
String
-
Sequence Similarities
-
GO - Molecular Function
-
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
Ipomoea nil
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Insertion Size
100-999 bp
Molecular Details of the Mutation
insertion of a 300-bp dTph1 transposon in the WDR gene in the first WD-repeat (321-bp downstream of the transcription start site)
Experimental Evidence
Authors
de Vetten N; Quattrocchio F; Mol J; Koes R
Abstract
In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the isolation of the an11 locus by transposon tagging. RNA gel blot experiments show that an11 is expressed independently from an1 and an2 throughout plant development, as well as in tissues that do not express the anthocyanin pathway. It encodes a novel WD-repeat protein that is highly conserved even in species that do not produce anthocyanins such as yeast, nematodes, and mammals. The observation that the human an11 homolog partially complements the an11 petunia mutant in transient assays shows that sequence similarity reflects functional conservation. Overexpression of an2 in an11- petals restored the activity of a structural anthocyanin gene in transient assays, indicating that AN11 acts upstream of AN2. Cell fractionation experiments show that the bulk of the AN11 protein is localized in the cytoplasm. Taken together, this indicates that AN11 is a cytoplasmic component of a conserved signal transduction cascade that modulates AN2 function in petunia, thereby linking cellular signals with transcriptional activation.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
COMMENTS
@TE - WDR1 is also named WDR and anthocyanin11 (an11).
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