GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002116
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Culex pipiens quinquefasciatus - sensitive
Trait State in Taxon B
Culex pipiens quinquefasciatus- resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
southern house mosquito
Synonyms
Culex fatigans; Culex pipiens fatigans; Culex pipiens quinquefasciatus; southern house mosquito; Culex fatigan; Culex pipiens quiquefasciatus; Culex quinquifasciatus; Culex quinquifasiatus; Culex quiquefasciatus
Rank
species
Lineage
Show more ... lata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Culicinae; Culicini; Culex; Culex; Culex pipiens complex
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
southern house mosquito
Synonyms
Culex fatigans; Culex pipiens fatigans; Culex pipiens quinquefasciatus; southern house mosquito; Culex fatigan; Culex pipiens quiquefasciatus; Culex quinquifasciatus; Culex quinquifasiatus; Culex quiquefasciatus
Rank
species
Lineage
Show more ... lata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Culicinae; Culicini; Culex; Culex; Culex pipiens complex
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
CYP9M10
Synonyms
Cyp9m10; Cyp9m10v2
String
-
Sequence Similarities
Belongs to the cytochrome P450 family.
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
Culex quinquefasciatus
GenebankID or UniProtKB
Mutation #1
Presumptive Null
No
Molecular Type
Aberration Type
Insertion Size
100-1000 kb
Molecular Details of the Mutation
increased expression of the gene - identical tandem duplications of a 100-kb sequence containing the CYP9M10 locus
Experimental Evidence
Authors
Itokawa K; Komagata O; Kasai S; Okamura Y; Masada M; Tomita T
Abstract
Development of insecticide resistance reduces the efficacy of controlling measures against the medical and agricultural insect pests. Cytochrome P450s are one of the major detoxification enzymes involved in insecticide metabolisms. Previously, we have reported that the P450 gene Cyp9m10 is about 260-fold overexpressed in a pyrethroid-resistant strain of Culex quinquefasciatus compared to a susceptible strain. In this study, we obtained direct evidence that the Cyp9m10 overexpression is caused by a cis-acting mutation. Additionally, a region of approximately 100 kb in length including the Cyp9m10 locus was specifically duplicated in the resistant strain. The two duplicated Cyp9m10 copies shared a completely identical sequence within the transcribed region and the flanking region up to the breakpoint located 1.1 kb upstream of the transcriptional start site. A Miniature Inverted-repeat Transposable Element (MITE)-like element was specifically inserted 0.2 kb upstream of both Cyp9m10 copies in the resistant strain. In backcross experiment, a haplotype containing the two duplicated Cyp9m10 copies was strongly associated with the pyrethroid resistance.

Copyright 2010 Elsevier Ltd. All rights reserved.
Mutation #2
Presumptive Null
No
Molecular Type
Aberration Type
SNP
Molecular Details of the Mutation
G27A - increased expression of the gene
Experimental Evidence
Authors
Itokawa K; Komagata O; Kasai S; Tomita T
Abstract
Although the importance of cis-acting mutations on detoxification enzyme genes for insecticide resistance is widely accepted, only a few of them have been determined as concrete mutations present in genomic DNA till date. The overexpression of a cytochrome P450 gene, CYP9M10, is associated with pyrethroid resistance in the southern house mosquito Culex quinquefasciatus. The haplotypes of CYP9M10 exhibiting overexpression (resistant haplotypes) belong to one specific phylogenetic lineage that shares high nucleotide sequence homology and the same insertion of a transposable element. Among the resistant haplotypes, allelic progression involving an additional cis-acting mutation and gene duplication evolved a CYP9M10 haplotype associated with extremely high transcription and strong pyrethroid resistance. Here we show that a single nucleotide substitution G-27A, which is located near the transcription start site of CYP9M10, is involved in the progression of the duplicated haplotype lineage. The deletion of a 7-bp AT-rich sequence that includes nucleotide -27 inhibited the initiation of transcription from the original transcriptional initiation site. The mutation was suspected to reside within a core promoter, TATA-box, of CYP9M10.

Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
@SelectiveSweep - There are only three segregating nucleotides within the region 2.2-kb upstream from the transcription start site: 2000T/G; 1176A/G and 27G/A. The G27A mutation apparently predates the duplication because they exist in both copies of duplicated haplotypes.
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