GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002117
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
rice pest Laodelphax striatellus - susceptible
Trait State in Taxon B
rice pest Laodelphax striatellus - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
small brown planthopper
Synonyms
Laodelphax striatella; small brown planthopper; Laodelphax striatella (Fallen, 1826); Laodelphax striatellus (Fallen, 1826)
Rank
species
Lineage
Show more ... Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Auchenorrhyncha; Fulgoromorpha; Fulgoroidea; Delphacidae; Criomorphinae; Laodelphax
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
small brown planthopper
Synonyms
Laodelphax striatella; small brown planthopper; Laodelphax striatella (Fallen, 1826); Laodelphax striatellus (Fallen, 1826)
Rank
species
Lineage
Show more ... Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Auchenorrhyncha; Fulgoromorpha; Fulgoroidea; Delphacidae; Criomorphinae; Laodelphax
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
CYP6FU1
Synonyms
-
String
-
Sequence Similarities
-
GO - Molecular Function
-
GO - Biological Process
-
UniProtKB
Laodelphax striatellus
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
Four cis-acting elements were identified whose influence on up-regulation was much more pronounced in combination than when present singly.
Experimental Evidence
Authors
Pu J; Sun H; Wang J; Wu M; Wang K; Denholm I; Han Z
Abstract
As well as arising from single point mutations in binding sites or detoxifying enzymes, it is likely that insecticide resistance mechanisms are frequently controlled by multiple genetic factors, resulting in resistance being inherited as a quantitative trait. However, empirical evidence for this is still rare. Here we analyse the causes of up-regulation of CYP6FU1, a monoxygenase implicated in resistance to deltamethrin in the rice pest Laodelphax striatellus. The 5'-flanking region of this gene was cloned and sequenced from individuals of a susceptible and a resistant strain. A luminescent reporter assay was used to evaluate different 5'-flanking regions and their fragments for promoter activity. Mutations enhancing promoter activity in various fragments were characterized, singly and in combination, by site mutation recovery. Nucleotide diversity in flanking sequences was greatly reduced in deltamethrin-resistant insects compared to susceptible ones. Phylogenetic sequence analysis found that CYP6FU1 had five different types of 5'-flanking region. All five types were present in a susceptible strain but only a single type showing the highest promoter activity was present in a resistant strain. Four cis-acting elements were identified whose influence on up-regulation was much more pronounced in combination than when present singly. Of these, two were new transcription factor (TF) binding sites produced by mutations, another one was also a new TF binding site alternated from an existing one, and the fourth was a unique transcription start site. These results demonstrate that multiple cis-acting elements are involved in up-regulating CYP6FU1 to generate a resistance phenotype.

Copyright © 2016 Elsevier Ltd. All rights reserved.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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