GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002438
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
tomato leafminer Tuta absoluta - susceptible
Trait State in Taxon B
tomato leafminer Tuta absoluta - resistant strain GR-IER-SD originating from Crete in Greece and obtained after four sequential selections of field populations with flubendiamide
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
Gnorimoschema absoluta; Phthorimaea absoluta; Scrobipalpula absoluta; Scrobipalpuloides absoluta; Tuta absoluta (Meyrick, 1917)
Rank
species
Lineage
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rustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Gelechioidea; Gelechiidae; Gelechiinae; Tuta
Parent
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
Gnorimoschema absoluta; Phthorimaea absoluta; Scrobipalpula absoluta; Scrobipalpuloides absoluta; Tuta absoluta (Meyrick, 1917)
Rank
species
Lineage
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rustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Gelechioidea; Gelechiidae; Gelechiinae; Tuta
Parent
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
RyR
Synonyms
Ryr; CG10844; D-RyR; Dmel\CG10844; DmRyR; DRR; dry; DRY; dRyr; dRyR; dya; l(2)k00424; l(2)k04913; Rya-44F; Rya-r4; rya-r44F; Rya-r44F; Rya-R44F; Rya-r76CD; ryr; RYR; RyRs
String
Sequence Similarities
Belongs to the ryanodine receptor (TC 1.A.3.1) family.
GO - Molecular Function
GO:0005509 : calcium ion binding
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GO - Biological Process
GO:0006874 : cellular calcium ion homeostasis
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GO - Cellular Component
GO:0016021 : integral component of membrane
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
G4903V corresponds to the G4946E mutation site shown to confer diamide resistance in diamondback moth
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | GGG | GTG | - |
Amino-acid | Gly | Val | 4946 |
Authors
Douris V; Papapostolou KM; Ilias A; Roditakis E; Kounadi S; Riga M; Nauen R; Vontas J
Abstract
Diamide insecticides are used widely against lepidopteran pests, acting as potent activators of insect Ryanodine Receptors (RyRs) and thus inducing muscle contraction and eventually death. However, resistant phenotypes have recently evolved in the field, associated with the emergence of target site resistance mutations (G4946E/V and I4790M). We investigated the frequency of the mutations found in a resistant population of Tuta absoluta from Greece (G4946V ~79% and I4790M ~21%) and the associated diamide resistance profile: there are very high levels of resistance against chlorantraniliprole (9329-fold) and flubendiamide (4969-fold), but moderate levels against cyantraniliprole (191-fold). To further investigate functionally the contribution of each mutation in the resistant phenotype, we used CRISPR/Cas9 to generate genome modified Drosophila carrying alternative allele combinations, and performed toxicity bioassays against all three diamides. Genome modified flies bearing the G4946V mutation exhibited high resistance ratios to flubendiamide (91.3-fold) and chlorantraniliprole (194.7-fold) when compared to cyantraniliprole (5.4-fold). Flies naturally wildtype for the I4790M mutation were moderately resistant to flubendiamide (15.3-fold) but significantly less resistant to chlorantraniliprole (7.5-fold), and cyantraniliprole (2.3-fold). These findings provide in vivo functional genetic confirmation for the role and relative contribution of RyR mutations in diamide resistance and suggest that the mutations confer subtle differences on the relative binding affinities of the three diamides at an overlapping binding site on the RyR protein.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Copyright © 2017 Elsevier Ltd. All rights reserved.
RELATED GEPHE
Related Genes
Related Haplotypes
EXTERNAL LINKS
COMMENTS
@Parallelism The two mutations found in GR-IER-SD have probably arisen independently since they are always found in different alleles and never in the same chromosome.
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