GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002486
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Heliothis virescens
Trait State in Taxon B
Heliothis virescens - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
tobacco budworm
Synonyms
tobacco budworm; Chloridea virescens; Helicoverpa virescens; Heliothis virescens (Fabricius, 1777)
Rank
species
Lineage
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poda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Heliothinae; Heliothis
Parent
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
tobacco budworm
Synonyms
tobacco budworm; Chloridea virescens; Helicoverpa virescens; Heliothis virescens (Fabricius, 1777)
Rank
species
Lineage
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poda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Heliothinae; Heliothis
Parent
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
para
Synonyms
bas; bss; CG9907; Dmel\CG9907; DmNav; DmNav1; DmNa[[v]]; DmNa[[V]]; DmNa[[v]]1; l(1)14Da; l(1)ESHS48; lincRNA.S9469; Nav1; Ocd; olfD; par; sbl; sbl-1; Shu; Shudderer
String
Sequence Similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Para subfamily.
GO - Molecular Function
GO:0005509 : calcium ion binding
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GO - Biological Process
GO - Cellular Component
GO:0005887 : integral component of plasma membrane
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Mutation #1
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
D1561V resulting from a GAC to GTC single base change and glutamic acid to glycine E1565G substitution resulting from a GAA to GGA single base change.
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | GAC | GTC | - |
Amino-acid | Asp | Val | 1561 |
Authors
Head DJ; McCaffery AR; Callaghan A
Abstract
Coding sequences from the intracellular segment linking repeat domains III and IV of the para-homologous sodium channel gene were amplified and cloned from two species of Heliothine larvae using RT-PCR techniques. Sequence comparisons between standard laboratory susceptible and nerve insensitive strains of the tobacco budworm (Heliothis virescens) and the cotton bollworm (Helicoverpa armigera) identified two mutations. These were an aspartic acid (GAC) to valine (GTC) and a glutamic acid (GAA) to glycine (GGA) mutation. PCR and sequencing was undertaken only on individuals that were demonstrated to be phenotypically nerve insensitive or susceptible to pyrethroids using a neurophysiological technique. The finding of identical mutations across two species suggests that they may be implicated in resistance.
Additional References
Mutation #2
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
D1561V resulting from a GAC to GTC single base change and glutamic acid to glycine E1565G substitution resulting from a GAA to GGA single base change.
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | GAA | GGA | - |
Amino-acid | Glu | Gly | 1565 |
Authors
Head DJ; McCaffery AR; Callaghan A
Abstract
Coding sequences from the intracellular segment linking repeat domains III and IV of the para-homologous sodium channel gene were amplified and cloned from two species of Heliothine larvae using RT-PCR techniques. Sequence comparisons between standard laboratory susceptible and nerve insensitive strains of the tobacco budworm (Heliothis virescens) and the cotton bollworm (Helicoverpa armigera) identified two mutations. These were an aspartic acid (GAC) to valine (GTC) and a glutamic acid (GAA) to glycine (GGA) mutation. PCR and sequencing was undertaken only on individuals that were demonstrated to be phenotypically nerve insensitive or susceptible to pyrethroids using a neurophysiological technique. The finding of identical mutations across two species suggests that they may be implicated in resistance.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
EXTERNAL LINKS
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