GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002515
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Ctenocephalides felis
Trait State in Taxon B
Ctenocephalides felis - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
cat flea
Synonyms
cat flea; Ctenocephalides felis (Bouche, 1835)
Rank
species
Lineage
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rthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Siphonaptera; Pulicomorpha; Pulicoidea; Pulicidae; Archaeopsyllinae; Ctenocephalides
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
cat flea
Synonyms
cat flea; Ctenocephalides felis (Bouche, 1835)
Rank
species
Lineage
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rthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Siphonaptera; Pulicomorpha; Pulicoidea; Pulicidae; Archaeopsyllinae; Ctenocephalides
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
para
Synonyms
bas; bss; CG9907; Dmel\CG9907; DmNav; DmNav1; DmNa[[v]]; DmNa[[V]]; DmNa[[v]]1; l(1)14Da; l(1)ESHS48; lincRNA.S9469; Nav1; Ocd; olfD; par; sbl; sbl-1; Shu; Shudderer
String
Sequence Similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Para subfamily.
GO - Molecular Function
GO:0005509 : calcium ion binding
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GO - Biological Process
GO - Cellular Component
GO:0005887 : integral component of plasma membrane
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
L1014F
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | Leu | Phe | 1014 |
Authors
Bass C; Schroeder I; Turberg A; M Field L; Williamson MS
Abstract
Knockdown resistance (kdr) to pyrethroid insecticides is caused by point mutations in the pyrethroid target site, the para-type sodium channel of nerve membranes. This most commonly involves alterations within the domain II (S4-S6) region of the channel protein where five different mutation sites have been identified across a range of insect species. To investigate the incidence of this mechanism in cat fleas, we have cloned and sequenced the IIS4-IIS6 region of the para sodium channel gene from seven laboratory flea strains. Analysis of these sequences revealed two amino acid replacements at residues previously implicated in pyrethroid resistance. One is the 'common' kdr mutation, a leucine to phenylalanine substitution (equivalent to L1014F of housefly) reported previously in several other insects. The other is a threonine to valine substitution (equivalent to T929V) and is a novel variant of the T929I mutation first identified in diamondback moth. The L1014F mutation was found at varying frequency in all of the laboratory flea strains, whereas the T929V mutation was found only in the highly resistant Cottontail strain. We have developed rapid PCR-based diagnostic assays for the detection of these mutations in individual cat fleas and used them to show that both L1014F and T929V are common in UK and US flea populations. This survey revealed a significant number of fleas that carry only the V929 allele indicating that co-expression with the F1014 allele is not necessary for flea viability.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
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