GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002547
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Tetranychus urticae
Trait State in Taxon B
Tetranychus urticae - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
two-spotted spider mite
Synonyms
two-spotted spider mite; red spider mite; twospotted mite; Tetranychus urticae Koch, 1836
Rank
species
Lineage
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ia; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
two-spotted spider mite
Synonyms
two-spotted spider mite; red spider mite; twospotted mite; Tetranychus urticae Koch, 1836
Rank
species
Lineage
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ia; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
para
Synonyms
bas; bss; CG9907; Dmel\CG9907; DmNav; DmNav1; DmNa[[v]]; DmNa[[V]]; DmNa[[v]]1; l(1)14Da; l(1)ESHS48; lincRNA.S9469; Nav1; Ocd; olfD; par; sbl; sbl-1; Shu; Shudderer
String
Sequence Similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Para subfamily.
GO - Molecular Function
GO:0005509 : calcium ion binding
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GO - Biological Process
GO - Cellular Component
GO:0005887 : integral component of plasma membrane
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
F1538I
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | - | - | - |
Authors
Tsagkarakou A; Van Leeuwen T; Khajehali J; Ilias A; Grispou M; Williamson MS; Tirry L; Vontas J
Abstract
We investigated pyrethroid resistance mechanisms in Tetranychus urticae strains from Greece. Combined bioassay, biochemical and synergistic data indicated that although P450 mono-oxygenase activities were associated with the trait, target site insensitivity was the major resistance component. A 3.3 kb cDNA fragment of the T. urticae para sodium channel gene encompassing segment 4 of domain II to segment 6 of domain IV was obtained by a degenerate PCR strategy. The T. urticae sequence showed highest identity (56%) to the scabies mite, Sarcoptes scabiei, and was phylogenetically classified within the divergent group of Arachnida. Comparison of resistant and susceptible strains identified the point mutation F1538I in segment 6 of domain III, which is known to confer strong resistance to pyrethroids, along with a second mutation (A1215D) in the intracellular linker connecting domains II and III with an unknown role. Three transcripts were identified corresponding to the k and l alternative exons. The mode of inheritance of resistance was confirmed as incompletely recessive, which is consistent with a target site mechanism for pyrethroids.
Additional References
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