GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002554
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Frankliniella occidentalis
Trait State in Taxon B
Frankliniella occidentalis - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
western flower thrips
Synonyms
Euthrips occidentalis; Frankliniella brunnescens; Frankliniella californica; Frankliniella occidentalis brunnescens; western flower thrips; Frankliniella occidentalis (Pergande, 1895); Frankliniella occidentalis brunnescens Priesner, 1932
Rank
species
Lineage
Show more ... ozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Thysanoptera; Terebrantia; Thripoidea; Thripidae; Thripinae; Frankliniella
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
western flower thrips
Synonyms
Euthrips occidentalis; Frankliniella brunnescens; Frankliniella californica; Frankliniella occidentalis brunnescens; western flower thrips; Frankliniella occidentalis (Pergande, 1895); Frankliniella occidentalis brunnescens Priesner, 1932
Rank
species
Lineage
Show more ... ozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Thysanoptera; Terebrantia; Thripoidea; Thripidae; Thripinae; Frankliniella
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
G275E in nAchR alpha 6 due to a single nucleotide change
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Puinean AM; Lansdell SJ; Collins T; Bielza P; Millar NS
Abstract
High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis, an economically important insect pest of vegetables, fruit and ornamental crops. We have cloned the nicotinic acetylcholine receptor (nAChR) α6 subunit from F. occidentalis (Foα6) and compared the nucleotide sequence of Foα6 from susceptible and spinosad-resistant insect populations (MLFOM and R1S respectively). A single nucleotide change has been identified in Foα6, resulting in the replacement of a glycine (G) residue in susceptible insects with a glutamic acid (E) in resistant insects. The resistance-associated mutation (G275E) is predicted to lie at the top of the third α-helical transmembrane domain of Foα6. Although there is no direct evidence identifying the location of the spinosad binding site, the analogous amino acid in the C. elegans glutamate-gated chloride channel lies in close proximity (4.4 Å) to the known binding site of ivermectin, another macrocyclic lactone pesticide. The functional consequences of the resistance-associated mutation have been examined in the human nAChR α7 subunit. Introduction of an analogous (A272E) mutation in α7 abolishes the modulatory effects of spinosad whilst having no significant effect upon activation by acetylcholine, consistent with spinosad having an allosteric mechanism of action.

© 2012 International Society for Neurochemistry.
RELATED GEPHE
Related Haplotypes
No matches found.
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