GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002556
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Trialeurodes vaporariorum - sensitive
Trait State in Taxon B
Trialeurodes vaporariorum - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
greenhouse whitefly
Synonyms
greenhouse whitefly; Trialeurodes vaporariorum (Westwood, 1856); Trialeurodes vaporarium
Rank
species
Lineage
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Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aleyrodoidea; Aleyrodidae; Aleyrodinae; Trialeurodes
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
greenhouse whitefly
Synonyms
greenhouse whitefly; Trialeurodes vaporariorum (Westwood, 1856); Trialeurodes vaporarium
Rank
species
Lineage
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Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aleyrodoidea; Aleyrodidae; Aleyrodinae; Trialeurodes
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
ACC
Synonyms
A1Z784_DROME; acc; Acc; ACoT; CG11198; CG8723; dACC; DmACC; Dmel\CG11198; FBgn0043811; Dmel_CG11198
String
-
Sequence Similarities
-
GO - Molecular Function
GO:0005524 : ATP binding
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GO - Biological Process
GO:0006633 : fatty acid biosynthetic process
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GO - Cellular Component
GO:0005737 : cytoplasm
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
E645K
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | Glu | Lys | 645 |
Authors
Karatolos N; Williamson MS; Denholm I; Gorman K; ffrench-Constant R; Nauen R
Abstract
Spiromesifen is a novel insecticide and is classed as a tetronic acid derivative. It targets the insects' acetyl-coenzyme A carboxylase (ACCase) enzyme, causing a reduction in lipid biosynthesis. At the time of this publication, there are no reports of resistance to this class of insecticides in insects although resistance has been observed in several mite species. The greenhouse whitefly Trialeurodes vaporariorum (Westwood) is a serious pest of protected vegetable and ornamental crops in temperate regions of the world and spiromesifen is widely used in its control. Mortality rates of UK and European populations of T. vaporariorum to spiromesifen were calculated and up to 26-fold resistance was found. We therefore sought to examine the molecular mechanism underlying spiromesifen resistance in this important pest. Pre-treatment with piperonyl butoxide did not synergize spiromesifen, suggesting a target-site resistance mechanism. The full length ACCase gene was sequenced for a range of T. vaporariorum strains and a strong association was found between spiromesifen resistance and a glutamic acid substitution with lysine in position 645 (E645K) of this gene. A TaqMan allelic discrimination assay confirmed these findings. Although this resistance is not considered sufficient to compromise the field performance of spiromesifen, this association of E645K with resistance is the first report of a potential target site mechanism affecting an ACCase inhibitor in an arthropod species.
© 2012 Rothamsted Research. Insect Molecular Biology © 2012 The Royal Entomological Society.
© 2012 Rothamsted Research. Insect Molecular Biology © 2012 The Royal Entomological Society.
RELATED GEPHE
Related Genes
Related Haplotypes
No matches found.
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