GEPHE SUMMARY Print
Entry Status
Published
GepheID
GP00002575
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Musca domestica - sensitive
Trait State in Taxon B
Drosophila melanogaster - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
house fly
Synonyms
house fly; Musca domestica Linnaeus, 1758
Rank
species
Lineage
Show more ... poda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Calyptratae; Muscoidea; Muscidae; Muscinae; Muscini; Musca; Musca
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
house fly
Synonyms
house fly; Musca domestica Linnaeus, 1758
Rank
species
Lineage
Show more ... poda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Calyptratae; Muscoidea; Muscidae; Muscinae; Muscini; Musca; Musca
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
UniProtKB
Drosophila melanogaster
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
I129V
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ile Val 129
Authors
Kozaki T; Shono T; Tomita T; Kono Y
Abstract
The cDNA of AChE in the housefly, Musca domestica, was sequenced and individual flies were genotyped by this gene in an inhibition assay of AChE activity with an organophaspate, fenitroxon. Mutations at Gly(342) and Tyr(407), which are reportedly conserved in resistant strains of Drosophila, were associated with the insensitivity to fenitroxon. Two other mutations, Ile(162) and Val(260), did not have an apparent effect on insensitivity. However, the four mutations are located in the active site of the enzyme, and therefore the non-neutral mutations in this gene are considered to cause the insensitivity of AChE in the development of insecticide resistance of the housefly.
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