GEPHE SUMMARY Print
Entry Status
Published
GepheID
GP00002576
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Bactrocera oleae - sensitive
Trait State in Taxon B
Bactrocera oleae - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
olive fruit fly
Synonyms
Bactrocera (Daculus) oleae; Bactrocera (Dacus) oleae; Dacus oleae; olive fruit fly; olive fly; Bactrocera oleae (Rossi, 1790)
Rank
species
Lineage
Show more ... a; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Tephritoidea; Tephritidae; Dacinae; Dacini; Bactrocera; Daculus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
olive fruit fly
Synonyms
Bactrocera (Daculus) oleae; Bactrocera (Dacus) oleae; Dacus oleae; olive fruit fly; olive fly; Bactrocera oleae (Rossi, 1790)
Rank
species
Lineage
Show more ... a; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Tephritoidea; Tephritidae; Dacinae; Dacini; Bactrocera; Daculus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
UniProtKB
Drosophila melanogaster
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
I129V
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ile Val 129
Authors
Vontas JG; Hejazi MJ; Hawkes NJ; Cosmidis N; Loukas M; Janes RW; Hemingway J
Abstract
A 2.2-kb full length cDNA containing an ORF encoding a putative acetylcholinesterase (AChE) precursor of 673 amino acid residues was obtained by a combined degenerate PCR and RACE strategy from an organophosphate-susceptible Bactrocera oleae strain. A comparison of cDNA sequences of individual insects from susceptible and resistant strains, coupled with an enzyme inhibition assay with omethoate, indicated a novel glycine-serine substitution (G488S), at an amino acid residue which is highly conserved across species (G396 of Torpedocalifornica AChE), as a likely cause of AChE insensitivity. This mutation was also associated with a 35-40% reduction in AChE catalytic efficiency. The I199V substitution, which confers low levels of resistance in Drosophila, was also present in B. oleae (I214V) and in combination with G488S produced up to a 16-fold decrease in insecticide sensitivity. This is the first agricultural pest where resistance has been associated with an alteration in AChE, which arises from point mutations located within the active site gorge of the enzyme.
RELATED GEPHE
Related Haplotypes
No matches found.
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