GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002604
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Tetranychus urticae - sensitive
Trait State in Taxon B
Tetranychus urticae - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
two-spotted spider mite
Synonyms
two-spotted spider mite; red spider mite; twospotted mite; Tetranychus urticae Koch, 1836
Rank
species
Lineage
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ia; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
two-spotted spider mite
Synonyms
two-spotted spider mite; red spider mite; twospotted mite; Tetranychus urticae Koch, 1836
Rank
species
Lineage
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ia; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
GluClalpha
Synonyms
BcDNA:HL07853; CG7535; CT23049; dGluCl-alpha; Dm-GluCl; Dmel\CG7535; DmGlu; DmGluClalpha; DrosGlu-Cl-alpha; DrosGluCl; DrosGluCl-alpha; DrosGluCl-alpha1; glc; glu; GluCl; GLUCL; GluCla; gluClalpha; GluClalpha1
String
Sequence Similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily.
GO - Molecular Function
GO:0004888 : transmembrane signaling receptor activity
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GO - Biological Process
GO:0007165 : signal transduction
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GO - Cellular Component
GO:0016021 : integral component of membrane
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
G323D in the highly conserved TM2 region of Tu_GluCl
Experimental Evidence
| Taxon A | Taxon B | Position | |
|---|---|---|---|
| Codon | - | - | - |
| Amino-acid | Gly | Glu | 326 |
Authors
Kwon DH; Yoon KS; Clark JM; Lee SH
Abstract
The molecular mechanisms and genetics of abamectin resistance mediated by target site insensitivity in the two-spotted spider mite, Tetranychus urticae, were investigated by comparing two isogenic abamectin-susceptible (AbaS) and abamectin-resistant (AbaR) strains. Cloning and sequencing of full-length cDNA fragments of gamma-amino butyric acid (GABA)-gated chloride channel genes revealed no polymorphisms between the two strains. However, sequence comparison of the full-length cDNA fragment of a T. urticae glutamate-gated chloride channel gene (TuGluCl) identified a G323D point mutation as being tentatively related with abamectin resistance. In individual F(2) progenies obtained by backcrossing, the G323D genotype was confirmed to correlate with abamectin resistance. Bioassays using progeny from reciprocal crossings revealed that the abamectin resistance trait resulting from TuGluCl insensitivity is incompletely recessive.
RELATED GEPHE
Related Genes
Related Haplotypes
EXTERNAL LINKS
COMMENTS
There are six orthologous GluCl genes in the genome of T. urticae.
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