GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002617
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Caenorhabditis elegans - sensitive
Trait State in Taxon B
Caenorhabditis elegans - resistant line after mutagenesis and selection
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
roundworm; Rhabditis elegans; Caenorhabditis elegans (Maupas, 1900); Rhabditis elegans Maupas, 1900
Rank
species
Lineage
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s; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
roundworm; Rhabditis elegans; Caenorhabditis elegans (Maupas, 1900); Rhabditis elegans Maupas, 1900
Rank
species
Lineage
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s; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
ACC
Synonyms
A1Z784_DROME; acc; Acc; ACoT; CG11198; CG8723; dACC; DmACC; Dmel\CG11198; FBgn0043811; Dmel_CG11198
String
-
Sequence Similarities
-
GO - Molecular Function
GO:0005524 : ATP binding
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GO - Biological Process
GO:0006633 : fatty acid biosynthetic process
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GO - Cellular Component
GO:0005737 : cytoplasm
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UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
A1559V
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | Ala | Val | - |
Main Reference
Authors
Guest M; Kriek N; Flemming AJ
Abstract
We have studied the mode of action of the insecticide spirotetramat in the nematode Caenorhabditis elegans. A combination of symptomology, forward genetics and genome editing show that spirotetramat acts on acetyl-CoA carboxylase (ACC) in C. elegans, as it does in insects. We found C. elegans embryos exposed to spirotetramat show a cell division defect which closely resembles the phenotype of loss-of-function mutations in the gene pod-2, which encodes ACC. We then identified two mutations in the carboxyl transferase domain of pod-2 (ACC) which confer resistance and were confirmed using CRISPR/Cas9. One of these mutations substitutes an invertebrate-specific amino acid with one ubiquitous in other taxa; this residue may, therefore, be a determinant of the selectivity of spirotetramat for invertebrates. Such a mutation may also be the target of selection for resistance in the field. Our study is a further demonstration of the utility of C. elegans in studying bioactive chemicals.
Copyright © 2020 Elsevier Inc. All rights reserved.
Copyright © 2020 Elsevier Inc. All rights reserved.
Additional References
RELATED GEPHE
Related Genes
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