GEPHE SUMMARY
Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002618
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Caenorhabditis elegans - sensitive
Trait State in Taxon B
Caenorhabditis elegans - resistant line after mutagenesis and selection
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
roundworm; Rhabditis elegans; Caenorhabditis elegans (Maupas, 1900); Rhabditis elegans Maupas, 1900
Rank
species
Lineage
Show more ...
s; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
roundworm; Rhabditis elegans; Caenorhabditis elegans (Maupas, 1900); Rhabditis elegans Maupas, 1900
Rank
species
Lineage
Show more ...
s; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
ACC
Synonyms
A1Z784_DROME; acc; Acc; ACoT; CG11198; CG8723; dACC; DmACC; Dmel\CG11198; FBgn0043811; Dmel_CG11198
String
-
Sequence Similarities
-
GO - Molecular Function
GO:0005524 : ATP binding
... show more
GO - Biological Process
GO:0006633 : fatty acid biosynthetic process
... show more
GO - Cellular Component
GO:0005737 : cytoplasm
... show more
UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
A1847V
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | Ala | Val | - |
Main Reference
Authors
Guest M; Kriek N; Flemming AJ
Abstract
We have studied the mode of action of the insecticide spirotetramat in the nematode Caenorhabditis elegans. A combination of symptomology, forward genetics and genome editing show that spirotetramat acts on acetyl-CoA carboxylase (ACC) in C. elegans, as it does in insects. We found C. elegans embryos exposed to spirotetramat show a cell division defect which closely resembles the phenotype of loss-of-function mutations in the gene pod-2, which encodes ACC. We then identified two mutations in the carboxyl transferase domain of pod-2 (ACC) which confer resistance and were confirmed using CRISPR/Cas9. One of these mutations substitutes an invertebrate-specific amino acid with one ubiquitous in other taxa; this residue may, therefore, be a determinant of the selectivity of spirotetramat for invertebrates. Such a mutation may also be the target of selection for resistance in the field. Our study is a further demonstration of the utility of C. elegans in studying bioactive chemicals.
Copyright © 2020 Elsevier Inc. All rights reserved.
Copyright © 2020 Elsevier Inc. All rights reserved.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
EXTERNAL LINKS
COMMENTS
A1559V apeared independently in two lines. A1847V also apeared independently in two lines.
YOUR FEEDBACK is welcome!