GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002630
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Plutella xylostella - sensitive
Trait State in Taxon B
Plutella xylostella - resistant in China
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
diamondback moth
Synonyms
diamondback moth; cabbage moth; Plutella xylostella (Linnaeus, 1758); Putella xylostella
Rank
species
Lineage
Show more ... a; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Yponomeutoidea; Plutellidae; Plutella
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
V1848I
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Val Ile 1881
Authors
Wang XL; Su W; Zhang JH; Yang YH; Dong K; Wu YD
Abstract
Indoxacarb and metaflumizone belong to a relatively new class of sodium channel blocker insecticides (SCBIs). Due to intensive use of indoxacarb, field-evolved indoxacarb resistance has been reported in several lepidopteran pests, including the diamondback moth Plutella xylostella, a serious pest of cruciferous crops. In particular, the BY12 population of P. xylostella, collected from Baiyun, Guangdong Province of China in 2012, was 750-fold more resistant to indoxacarb and 70-fold more resistant to metaflumizone compared with the susceptible Roth strain. Comparison of complementary DNA sequences encoding the sodium channel genes of Roth and BY12 revealed two point mutations (F1845Y and V1848I) in the sixth segment of domain IV of the PxNav protein in the BY population. Both mutations are located within a highly conserved sequence region that is predicted to be involved in the binding sites of local anesthetics and SCBIs based on mammalian sodium channels. A significant correlation was observed among 10 field-collected populations between the mutant allele (Y1845 or I1848) frequencies (1.7% to 52.5%) and resistance levels to both indoxacarb (34- to 870-fold) and metaflumizone (1- to 70-fold). The two mutations were never found to co-exist in the same allele of PxNav , suggesting that they arose independently. This is the first time that sodium channel mutations have been associated with high levels of resistance to SCBIs. F1845Y and V1848I are molecular markers for resistance monitoring in the diamondback moth and possibly other insect pest species.

© 2015 Institute of Zoology, Chinese Academy of Sciences.
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