GEPHE SUMMARY
Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002631
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Tuta absoluta - sensitive
Trait State in Taxon B
Tuta absoluta - resistant from Greece
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
Gnorimoschema absoluta; Phthorimaea absoluta; Scrobipalpula absoluta; Scrobipalpuloides absoluta; Tuta absoluta (Meyrick, 1917)
Rank
species
Lineage
Show more ...
rustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Gelechioidea; Gelechiidae; Gelechiinae; Tuta
Parent
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
Gnorimoschema absoluta; Phthorimaea absoluta; Scrobipalpula absoluta; Scrobipalpuloides absoluta; Tuta absoluta (Meyrick, 1917)
Rank
species
Lineage
Show more ...
rustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Gelechioidea; Gelechiidae; Gelechiinae; Tuta
Parent
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
para
Synonyms
bas; bss; CG9907; Dmel\CG9907; DmNav; DmNav1; DmNa[[v]]; DmNa[[V]]; DmNa[[v]]1; l(1)14Da; l(1)ESHS48; lincRNA.S9469; Nav1; Ocd; olfD; par; sbl; sbl-1; Shu; Shudderer
String
Sequence Similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Para subfamily.
GO - Molecular Function
GO:0005509 : calcium ion binding
... show more
GO - Biological Process
GO - Cellular Component
GO:0005887 : integral component of plasma membrane
... show more
UniProtKB
Drosophila melanogaster
Drosophila melanogaster
Presumptive Null
Molecular Type
Aberration Type
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
V1848I
Experimental Evidence
Taxon A | Taxon B | Position | |
---|---|---|---|
Codon | - | - | - |
Amino-acid | Val | Ile | 1881 |
Authors
Roditakis E; Mavridis K; Riga M; Vasakis E; Morou E; Rison JL; Vontas J
Abstract
Indoxacarb is an important active ingredient extensively used for the control of Tuta absoluta, a major tomato pest, playing a particular role in insecticide resistance management schemes.
Reduced susceptibility to indoxacarb was identified (1794-fold resistance) through toxicological bioassays in a field population from Greece and evolved rapidly to resistance after short laboratory selection. Combined bioassays with synergists and biochemical analysis suggested only a partial involvement of detoxification enzymes in the resistant phenotype. To investigate the role of target-site resistance, segment 6 of domain IV of the sodium channel in T. absoluta was cloned and the sequences compared between susceptible and indoxacarb-resistant T. absoluta insects. The presence of the F1845Y and the V1848I indoxacarb resistance mutations was detected and was strongly associated with the phenotype. These amino acid substitutions correspond to recently characterised indoxacarb resistance mutations in diamondback moth (Plutella xylostella). Robust and accurate PCR-RFLP assays were subsequently developed and successfully validated for detecting both indoxacarb resistance mutations in field T. absoluta populations.
The identification of indoxacarb resistance mutations and the development of diagnostic tools will allow early detection of indoxacarb resistance, facilitating implementation of appropriate resistance management strategies, thus delaying the spread of resistance. © 2016 Society of Chemical Industry.
© 2016 Society of Chemical Industry.
Reduced susceptibility to indoxacarb was identified (1794-fold resistance) through toxicological bioassays in a field population from Greece and evolved rapidly to resistance after short laboratory selection. Combined bioassays with synergists and biochemical analysis suggested only a partial involvement of detoxification enzymes in the resistant phenotype. To investigate the role of target-site resistance, segment 6 of domain IV of the sodium channel in T. absoluta was cloned and the sequences compared between susceptible and indoxacarb-resistant T. absoluta insects. The presence of the F1845Y and the V1848I indoxacarb resistance mutations was detected and was strongly associated with the phenotype. These amino acid substitutions correspond to recently characterised indoxacarb resistance mutations in diamondback moth (Plutella xylostella). Robust and accurate PCR-RFLP assays were subsequently developed and successfully validated for detecting both indoxacarb resistance mutations in field T. absoluta populations.
The identification of indoxacarb resistance mutations and the development of diagnostic tools will allow early detection of indoxacarb resistance, facilitating implementation of appropriate resistance management strategies, thus delaying the spread of resistance. © 2016 Society of Chemical Industry.
© 2016 Society of Chemical Industry.
RELATED GEPHE
Related Genes
Related Haplotypes
EXTERNAL LINKS
COMMENTS
YOUR FEEDBACK is welcome!