GEPHE SUMMARY Print
Gephebase Gene
RYR
Entry Status
Published
GepheID
GP00002633
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Spodoptera exigua - susceptible
Trait State in Taxon B
Spodoptera exigua - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
beet armyworm
Synonyms
beet armyworm; pigweed caterpillar; small mottled willow caterpillar; Spodoptera exigua (Hubner, 1808)
Rank
species
Lineage
Show more ... da; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Amphipyrinae; Spodoptera
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
beet armyworm
Synonyms
beet armyworm; pigweed caterpillar; small mottled willow caterpillar; Spodoptera exigua (Hubner, 1808)
Rank
species
Lineage
Show more ... da; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Amphipyrinae; Spodoptera
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
I4743M - corresponds to I4790M in PxRyR
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ile Met 4790
Authors
Zuo YY; Ma HH; Lu WJ; Wang XL; Wu SW; Nauen R; Wu YD; Yang YH
Abstract
Insect ryanodine receptors (RyRs) are the targets of diamide insecticides. Two point mutations G4946E and I4790M (numbering according to Plutella xylostella, PxRyR) in the transmembrane domain of the insect RyRs associated with diamide resistance have so far been identified in three lepidopteran pests, P. xylostella, Tuta absoluta and Chilo suppressalis. In this study, we identified one of the known RyR target site resistance mutations (I4790M) in a field-collected population of Spodoptera exigua. The field-collected WF population of S. exigua exhibited 154 fold resistance to chlorantraniliprole when compared with the susceptible WH-S strain. Sequencing the transmembrane domains of S. exigua RyR (SeRyR) revealed that the resistant WF strain was homozygous for the I4743M mutation (corresponding to I4790M in PxRyR), whereas the G4900E allele (corresponding to G4946E of PxRyR) was not detected. The 4743M allele was introgressed into the susceptible WH-S strain by crossing WF with WH-S, followed by three rounds of backcrossing with WH-S. The introgressed strain 4743M was homozygous for the mutant 4743M allele and shared about 94% of its genetic background with that of the recipient WH-S strain. Compared with WH-S, the near-isogenic 4743M strain showed moderate levels of resistance to chlorantraniliprole (21 fold), cyantraniliprole (25 fold) and flubendiamide (22 fold), suggesting that the I4743M mutation confers medium levels of resistance to all three diamides. Genetic analysis showed diamide resistance in the 4743M strain was inherited as an autosomal and recessive trait. Results from this study have direct implications for the design of appropriate resistance monitoring and management practices to sustainably control S. exigua.

© 2019 Institute of Zoology, Chinese Academy of Sciences.
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