GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002654
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Rhyzopertha dominica - sensitive
Trait State in Taxon B
Rhyzopertha dominica - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
lesser grain borer
Synonyms
lesser grain borer; Rhyzopertha dominica (Fabricius, 1792); Rhizopertha dominica
Rank
species
Lineage
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Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Bostrichiformia; Bostrichoidea; Bostrichidae; Dinoderinae; Rhyzopertha
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
lesser grain borer
Synonyms
lesser grain borer; Rhyzopertha dominica (Fabricius, 1792); Rhizopertha dominica
Rank
species
Lineage
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Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Bostrichiformia; Bostrichoidea; Bostrichidae; Dinoderinae; Rhyzopertha
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
CHRNA6
Synonyms
CHNRA6
String
Sequence Similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-6/CHRNA6 sub-subfamily.
GO - Molecular Function
GO:0015464 : acetylcholine receptor activity
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GO - Biological Process
GO:0007165 : signal transduction
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GO - Cellular Component
GO:0005886 : plasma membrane
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UniProtKB
Homo sapiens
Homo sapiens
Presumptive Null
Molecular Type
Aberration Type
Deletion Size
unknown
Molecular Details of the Mutation
Three mutations were found in the resistant strain compared with the susceptible one: (1) a 181-bp fragment truncated at the N-terminus resulting in the appearance of a premature stop codon - (2) one missing bp at the position 997 causing a frame-shift mutation and (3) an 87-bp fragment truncated in the TM2 region.
Experimental Evidence
Authors
Wang HT; Tsai CL; Chen ME
Abstract
The lesser grain borer, Rhyzopertha dominica, which is a primary pest of stored products, breaks up whole grains and makes them susceptible to secondary infestation by other pests. Insecticide application is the main control measure against this borer. A resistant strain of R. dominica against the insecticide, spinosad, was selected in the laboratory. The full-length cDNA of the target site of spinosad, nicotinic acetylcholine receptor subunit α6, from R. dominica (Rdα6) was cloned and analyzed using reverse transcription PCR and rapid amplification of cDNA ends. The complete 2133-bp cDNA contains the open reading frame of 1497 bp encoding a 498-amino-acid protein. There are four predicted transmembrane (TM) regions, and six extracellular ligand-binding sites at the N-terminus, upstream from the first TM in Rdα6. Three mutations have been found in the resistant strain compared with the susceptible one: (1) a 181-bp fragment truncated at the N-terminus, resulting in the appearance of a premature stop codon, (2) one missing bp at the position 997, causing a frame-shift mutation, and (3) an 87-bp fragment truncated in the TM2 region. In addition, real-time quantitative PCR was applied to detect the transcriptional expression of Rdα6 in both the susceptible and resistant strains. The results indicated that the expression of Rdα6 was significantly lower in then resistant strain than in susceptible one. In conclusion, mutation of Rdα6 may cause R. dominica resistant to spinosad due to target site insensitivity.
Copyright © 2018 Elsevier Inc. All rights reserved.
Copyright © 2018 Elsevier Inc. All rights reserved.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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