GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000291
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Culex pipiens quinquefasciatus - sensitive S-LAB
Trait State in Taxon B
Culex pipiens quinquefasciatus - resistant Tem-R
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
southern house mosquito
Synonyms
Culex fatigans; Culex pipiens fatigans; Culex pipiens quinquefasciatus; southern house mosquito; Culex fatigan; Culex pipiens quiquefasciatus; Culex quinquifasciatus; Culex quinquifasiatus; Culex quiquefasciatus
Rank
species
Lineage
Show more ... lata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Culicinae; Culicini; Culex; Culex; Culex pipiens complex
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Culex pipiens quinquefasciatus - sensitive S-LAB
Taxon B
Common Name
southern house mosquito
Synonyms
Culex fatigans; Culex pipiens fatigans; Culex pipiens quinquefasciatus; southern house mosquito; Culex fatigan; Culex pipiens quiquefasciatus; Culex quinquifasciatus; Culex quinquifasiatus; Culex quiquefasciatus
Rank
species
Lineage
Show more ... lata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Culicinae; Culicini; Culex; Culex; Culex pipiens complex
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Culex pipiens quinquefasciatus - resistant Tem-R
GENOTYPIC CHANGE
Generic Gene Name
B1
Synonyms
-
String
-
Sequence Similarities
Belongs to the type-B carboxylesterase/lipase family.
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
Culex pipiens
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
Amplification of a region containing both genes
Experimental Evidence
Authors
Rooker S; Guillemaud T; Bergé J; Pasteur N; Raymond M
Abstract
In Culex pipiens mosquitoes, resistance to organophosphorous insecticides often results from increased detoxification by two types of esterases, A and B, which are closely linked. Overproduction of all esterase B so far investigated (B1, B2, B4, B5 and B6) is from gene amplification. An esterase A gene (esterase A2) has recently been cloned from mosquitoes with the overproduced esterases A2 and B2, and amplification of this gene has also been reported. We describe the cDNA sequences of three additional esterase genes from insecticide-resistant strains of Culex pipiens originating from France and California which show at least 93 per cent homology with the esterase A2 gene sequence. Restriction enzyme mapping shows that the esterase A gene lies within 2.2 kb of the esterase B gene. In mosquitoes with overproduced esterases A2 and B2, the amplification level of esterase A is equal to that of esterase B suggesting that the genes are coamplified. Furthermore, we show that in one strain with an overproduced A esterase (A1), gene amplification cannot account for the increased protein level. This indicates that overproduction of esterases A can be achieved through two different mechanisms: gene amplification and a regulatory mechanism--the nature of which remains to be identified.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
COMMENTS
Cluster of paralogous genes
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