GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000293
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Myzus persicae - sensitive
Trait State in Taxon B
Myzus persicae- resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
green peach aphid
Synonyms
Myzus (Nectarosiphon) persicae; green peach aphid; peach-potato aphid; Myzus persicae (Sulzer, 1776); Myzus persiceae
Rank
species
Lineage
Show more ... Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae; Aphidinae; Macrosiphini; Myzus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
green peach aphid
Synonyms
Myzus (Nectarosiphon) persicae; green peach aphid; peach-potato aphid; Myzus persicae (Sulzer, 1776); Myzus persiceae
Rank
species
Lineage
Show more ... Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae; Aphidinae; Macrosiphini; Myzus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
-
Synonyms
-
String
-
Sequence Similarities
Belongs to the type-B carboxylesterase/lipase family.
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
Myzus persicae
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
Whole gene amplification. Amplification of the E4 gene is closely linked to a chromosomal translocation (the autosomal 1-3 translocation event) and amplified genes are situated at a single heterozygous site on autosome 3 as a tandem array of head-to-tail amplicons.
Experimental Evidence
Authors
Field LM; Devonshire AL; Forde BG
Abstract
cDNA clones for the esterase (E4) responsible for broad insecticide resistance in peach-potato aphids (Myzus persicae Sulz.) were isolated and used to study the molecular basis of resistance. Increased esterase synthesis by resistant aphids was found to be associated with amplification of the structural gene for the esterase (E4 or its closely related variant, FE4), the degree of amplification being correlated with the activity of the esterase and the level of resistance. Hybridization of the cDNA clones to genomic Southern blots showed that only some of the esterase-related restriction fragments are amplified. Qualitative differences between restriction patterns in different clones of resistant aphids correlated with the presence or absence of a specific chromosome translocation and with production of E4 or FE4.
EXTERNAL LINKS
COMMENTS
The esterase E4 gene can be found in different copy numbers and at different sites around the genome. @Epimutation - Resistance resulting from amplified E4 genes can be unstable with ‘revertant’ clones displaying a sudden loss of both esterase gene expression and insecticide resistance within a single generation. In revertants the large reduction in expression is associated with the loss of 5-methylcytosine (5 mC) present in CpG doublets within the E4 genes. This results in gene silencing via demethylation.
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