GEPHE SUMMARY Print
Entry Status
Published
GepheID
GP00000327
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Pisum sativum- line JI15
Trait State in Taxon B
Pisum sativum - b type line JI73 and others
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
pea
Synonyms
pea; garden pea; peas; Pisum sativum L.
Rank
species
Lineage
Show more ... Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Pisum sativum- line JI15
Taxon B
Latin Name
Common Name
pea
Synonyms
pea; garden pea; peas; Pisum sativum L.
Rank
species
Lineage
Show more ... Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Pisum sativum - b type line JI73 and others
GENOTYPIC CHANGE
UniProtKB
Solanum melongena
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Deletion Size
10-99 bp
Molecular Details of the Mutation
23bp deletion reuslting in frameshift and truncated protein
Experimental Evidence
Authors
Moreau C; Ambrose MJ; Turner L; Hill L; Ellis TH; Hofer JM
Abstract
The inheritance of flower color in pea (Pisum sativum) has been studied for more than a century, but many of the genes corresponding to these classical loci remain unidentified. Anthocyanins are the main flower pigments in pea. These are generated via the flavonoid biosynthetic pathway, which has been studied in detail and is well conserved among higher plants. A previous proposal that the Clariroseus (B) gene of pea controls hydroxylation at the 5' position of the B ring of flavonoid precursors of the anthocyanins suggested to us that the gene encoding flavonoid 3',5'-hydroxylase (F3'5'H), the enzyme that hydroxylates the 5' position of the B ring, was a good candidate for B. In order to test this hypothesis, we examined mutants generated by fast neutron bombardment. We found allelic pink-flowered b mutant lines that carried a variety of lesions in an F3'5'H gene, including complete gene deletions. The b mutants lacked glycosylated delphinidin and petunidin, the major pigments present in the progenitor purple-flowered wild-type pea. These results, combined with the finding that the F3'5'H gene cosegregates with b in a genetic mapping population, strongly support our hypothesis that the B gene of pea corresponds to a F3'5'H gene. The molecular characterization of genes involved in pigmentation in pea provides valuable anchor markers for comparative legume genomics and will help to identify differences in anthocyanin biosynthesis that lead to variation in pigmentation among legume species.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
1
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