GEPHE SUMMARY
Entry Status
Published
GepheID
GP00000038
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Bactrocera oleae- sensitive
Trait State in Taxon B
Bactrocera oleae - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
olive fruit fly
Synonyms
Bactrocera (Daculus) oleae; Bactrocera (Dacus) oleae; Dacus oleae; olive fruit fly; olive fly; Bactrocera oleae (Rossi, 1790)
Rank
species
Lineage
Show more ... a; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Tephritoidea; Tephritidae; Dacinae; Dacini; Bactrocera; Daculus
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
olive fruit fly
Synonyms
Bactrocera (Daculus) oleae; Bactrocera (Dacus) oleae; Dacus oleae; olive fruit fly; olive fly; Bactrocera oleae (Rossi, 1790)
Rank
species
Lineage
Show more ... a; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Tephritoidea; Tephritidae; Dacinae; Dacini; Bactrocera; Daculus
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
Deletion Size
1-9 bp
Molecular Details of the Mutation
9bp deletion of three glutamine residues at positions 642_644
Experimental Evidence
Authors
Kakani EG; Ioannides IM; Margaritopoulos JT; Seraphides NA; Skouras PJ; Tsitsipis JA; Mathiopoulos KD
Abstract
Organophosphate resistance in the olive fly was previously shown to associate with two point mutations in the ace gene. The frequency of these mutations was monitored in Bactrocera oleae individuals of increasing resistance. In spite of the difference in resistance among the individuals, there was no correlation between mutation frequencies and resistance level, indicating that other factors may contribute to this variation. The search for additional mutations in the ace gene of highly resistant insects revealed a small deletion at the carboxyl terminal of the protein (termed Delta3Q). Significant correlation was shown between the mutation frequency and resistance level in natural populations. In addition, remaining activity of acetylcholinesterase enzyme (AChE) after dimethoate inhibition was higher in genotypes carrying the mutation. These results strongly suggest a role of Delta3Q in high levels of organophosphate (OP) resistance. Interestingly, the carboxyl terminal of AChE is normally cleaved and substituted by a glycosylphosphatidylinositol (GPI) anchor. We hypothesize that Delta3Q may improve GPI anchoring, thus increasing the amount of AChE that reaches the synaptic cleft. In this way, despite the presence of insecticide, enough enzyme would remain in the cleft for its normal role of acetylcholine hydrolysis, allowing the insect to survive. This provides a previously un-described mechanism of resistance.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
1
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