GEPHE SUMMARY
Gephebase Gene
GW2
Entry Status
Published
GepheID
GP00000425
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait
Trait State in Taxon A
Triticum aestivum - Chinese spring
Trait State in Taxon B
Triticum aestivum - Lankaodali
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
bread wheat
Synonyms
Triticum aestivum subsp. aestivum; Triticum vulgare; bread wheat; Canadian hard winter wheat; common wheat; wheat; Triticum aestivum L.; Triticum vulgare L.; Triticum vulgare Vill., nom. illeg.; Tricum aestivum; Triticum aestivam; Triticum aestivum8
Rank
species
Lineage
Show more ... a; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Triticum aestivum - Chinese spring
Taxon B
Common Name
bread wheat
Synonyms
Triticum aestivum subsp. aestivum; Triticum vulgare; bread wheat; Canadian hard winter wheat; common wheat; wheat; Triticum aestivum L.; Triticum vulgare L.; Triticum vulgare Vill., nom. illeg.; Tricum aestivum; Triticum aestivam; Triticum aestivum8
Rank
species
Lineage
Show more ... a; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Triticum aestivum - Lankaodali
GENOTYPIC CHANGE
UniProtKB
Oryza sativa subsp. indica
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Insertion Size
1-9 bp
Molecular Details of the Mutation
1bp insertion resulting in premature stop codon
Experimental Evidence
Authors
Yang Z; Bai Z; Li X; Wang P; Wu Q; Yang L; Li L; Li X
Abstract
TaGW2, an orthologous gene of rice OsGW2, has been associated with kernel width and weight of bread wheat (Triticum aestivum). Difference in TaGW2 coding sequence was not found among different wheat varieties in previous researches. In this study, we found eight exons and seven introns in TaGW2 with a full-length cDNA sequence of 1,275 bp, which contains a conserved function domain and seven splice sites that shared homology with rice OsGW2. A single T-base insertion in the eighth exon of TaGW2 on chromosome 6A was detected in a large-kernel wheat variety, Lankaodali. This insertion mutation reduces the coding protein sequence from normal 424 amino acids (~47.2 kDa) to 328 amino acids (~37.1 kDa) by truncating 96 amino acids. The result was validated by identifying histidine-tagged TaGW2 proteins encoded by both alleles of the mutant and the wild types in SDS-PAGE. Allele-specific PCR markers were developed based on the single nucleotide polymorphism (SNP) site. The SNP markers were genotyped for an F(2) segregation population from the cross of Lankaodali × Chinese Spring. Seed traits of F(2:3) families were evaluated in three different environments. The association analysis indicated that F(2:3) families with the mutated TaGW2 allele significantly increased kernel width (KW) and thousand-kernel weight (TKW), and slightly improved kernel length (KL). Using the SNP markers, another two varieties harbored the mutated TaGW2 allele were successfully identified from 22 additional wheat varieties, and they both have large KW and TKW. Cloning and sequencing of the gene further confirmed the functions of the mutated allele of TaGW2 in the two large kernel varieties. The results suggested that TaGW2 may negatively regulate kernel size variation, which shares the same function as OsGW2 in rice. The successful development of SNP markers provides a useful tool for improving kernel yield in wheat.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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